2r68
From Proteopedia
(New page: '''Unreleased structure''' The entry 2r68 is ON HOLD until Paper Publication Authors: Sivaraman, J., Chua, T.K. Description: Complex Structure of Sucrose Phosphate Synthase (SPS)-S6P o...) |
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| + | {{STRUCTURE_2r68| PDB=2r68 | SCENE= }} | ||
| - | + | '''Complex Structure of Sucrose Phosphate Synthase (SPS)-S6P of Halothermothrix orenii''' | |
| - | Description: Complex Structure of Sucrose Phosphate Synthase (SPS)-S6P of Halothermothrix orenii | ||
| + | ==Overview== | ||
| + | Sucrose phosphate synthase (SPS) catalyzes the transfer of a glycosyl group from an activated donor sugar, such as uridine diphosphate glucose (UDP-Glc), to a saccharide acceptor d-fructose 6-phosphate (F6P), resulting in the formation of UDP and d-sucrose-6'-phosphate (S6P). This is a central regulatory process in the production of sucrose in plants, cyanobacteria, and proteobacteria. Here, we report the crystal structure of SPS from the nonphotosynthetic bacterium Halothermothrix orenii and its complexes with the substrate F6P and the product S6P. SPS has two distinct Rossmann-fold domains with a large substrate binding cleft at the interdomain interface. Structures of two complexes show that both the substrate F6P and the product S6P bind to the A-domain of SPS. Based on comparative analysis of the SPS structure with other related enzymes, the donor substrate, nucleotide diphosphate glucose, binds to the B-domain of SPS. Furthermore, we propose a mechanism of catalysis by H. orenii SPS. Our findings indicate that SPS from H. orenii may represent a valid model for the catalytic domain of plant SPSs and thus may provide useful insight into the reaction mechanism of the plant enzyme. | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 | + | ==About this Structure== |
| + | 2R68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halothermothrix_orenii_h_168 Halothermothrix orenii h 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R68 OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | The Structure of Sucrose Phosphate Synthase from Halothermothrix orenii Reveals Its Mechanism of Action and Binding Mode., Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J, Plant Cell. 2008 Apr;20(4):1059-72. Epub 2008 Apr 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18424616 18424616] | ||
| + | [[Category: Halothermothrix orenii h 168]] | ||
| + | [[Category: Single protein]] | ||
| + | [[Category: Sucrose-phosphate synthase]] | ||
| + | [[Category: Chua, T K.]] | ||
| + | [[Category: Sivaraman, J.]] | ||
| + | [[Category: Rossmann-fold]] | ||
| + | [[Category: Transferase]] | ||
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:44:25 2008'' | ||
Revision as of 07:44, 11 June 2008
Complex Structure of Sucrose Phosphate Synthase (SPS)-S6P of Halothermothrix orenii
Overview
Sucrose phosphate synthase (SPS) catalyzes the transfer of a glycosyl group from an activated donor sugar, such as uridine diphosphate glucose (UDP-Glc), to a saccharide acceptor d-fructose 6-phosphate (F6P), resulting in the formation of UDP and d-sucrose-6'-phosphate (S6P). This is a central regulatory process in the production of sucrose in plants, cyanobacteria, and proteobacteria. Here, we report the crystal structure of SPS from the nonphotosynthetic bacterium Halothermothrix orenii and its complexes with the substrate F6P and the product S6P. SPS has two distinct Rossmann-fold domains with a large substrate binding cleft at the interdomain interface. Structures of two complexes show that both the substrate F6P and the product S6P bind to the A-domain of SPS. Based on comparative analysis of the SPS structure with other related enzymes, the donor substrate, nucleotide diphosphate glucose, binds to the B-domain of SPS. Furthermore, we propose a mechanism of catalysis by H. orenii SPS. Our findings indicate that SPS from H. orenii may represent a valid model for the catalytic domain of plant SPSs and thus may provide useful insight into the reaction mechanism of the plant enzyme.
About this Structure
2R68 is a Single protein structure of sequence from Halothermothrix orenii h 168. Full crystallographic information is available from OCA.
Reference
The Structure of Sucrose Phosphate Synthase from Halothermothrix orenii Reveals Its Mechanism of Action and Binding Mode., Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J, Plant Cell. 2008 Apr;20(4):1059-72. Epub 2008 Apr 18. PMID:18424616 Page seeded by OCA on Wed Jun 11 10:44:25 2008
