2z9i
From Proteopedia
(New page: '''Unreleased structure''' The entry 2z9i is ON HOLD until Paper Publication Authors: Palaninathan, S.K., Mohamedmohaideen, N.N., Sacchettini, J.C. Description: Crystal structure of RV...) |
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| - | + | [[Image:2z9i.jpg|left|200px]] | |
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| + | {{STRUCTURE_2z9i| PDB=2z9i | SCENE= }} | ||
| - | + | '''Crystal structure of RV0983 from Mycobacterium tuberculosis- Proteolytically active form''' | |
| - | Description: Crystal structure of RV0983 from Mycobacterium tuberculosis-Proteolytically active form | ||
| + | ==Overview== | ||
| + | The high-temperature requirement A (HtrA) family of serine proteases has been shown to play an important role in the environmental and cellular stress damage control system in Escherichia coli. Mycobacterium tuberculosis ( Mtb) has three putative HtrA-like proteases, HtrA1, HtrA2, and HtrA3. The deletion of htrA2 gives attenuated virulence in a mouse model of TB. Biochemical analysis reveals that HtrA2 can function both as a protease and as a chaperone. The three-dimensional structure of HtrA2 determined at 2.0 A resolution shows that the protease domains form the central core of the trimer and the PDZ domains extend to the periphery. Unlike E. coli DegS and DegP, the protease is naturally active due to the formation of the serine protease-like catalytic triad and its uniquely designed oxyanion hole. Both protease and PDZ binding pockets of each HtrA2 molecule are occupied by autoproteolytic peptide products and reveal clues for a novel autoregulatory mechanism that might have significant importance in HtrA-associated virulence of Mtb. | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 | + | ==About this Structure== |
| + | 2Z9I is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z9I OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Structure and function of the virulence-associated high-temperature requirement A of Mycobacterium tuberculosis., Mohamedmohaideen NN, Palaninathan SK, Morin PM, Williams BJ, Braunstein M, Tichy SE, Locker J, Russell DH, Jacobs WR Jr, Sacchettini JC, Biochemistry. 2008 Jun 10;47(23):6092-102. Epub 2008 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18479146 18479146] | ||
| + | [[Category: Mycobacterium tuberculosis]] | ||
| + | [[Category: Protein complex]] | ||
| + | [[Category: Mohamedmohaideen, N N.]] | ||
| + | [[Category: Palaninathan, S K.]] | ||
| + | [[Category: Sacchettini, J C.]] | ||
| + | [[Category: Htra]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Serine protease]] | ||
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:46:02 2008'' | ||
Revision as of 07:46, 11 June 2008
Crystal structure of RV0983 from Mycobacterium tuberculosis- Proteolytically active form
Overview
The high-temperature requirement A (HtrA) family of serine proteases has been shown to play an important role in the environmental and cellular stress damage control system in Escherichia coli. Mycobacterium tuberculosis ( Mtb) has three putative HtrA-like proteases, HtrA1, HtrA2, and HtrA3. The deletion of htrA2 gives attenuated virulence in a mouse model of TB. Biochemical analysis reveals that HtrA2 can function both as a protease and as a chaperone. The three-dimensional structure of HtrA2 determined at 2.0 A resolution shows that the protease domains form the central core of the trimer and the PDZ domains extend to the periphery. Unlike E. coli DegS and DegP, the protease is naturally active due to the formation of the serine protease-like catalytic triad and its uniquely designed oxyanion hole. Both protease and PDZ binding pockets of each HtrA2 molecule are occupied by autoproteolytic peptide products and reveal clues for a novel autoregulatory mechanism that might have significant importance in HtrA-associated virulence of Mtb.
About this Structure
2Z9I is a Protein complex structure of sequences from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
Structure and function of the virulence-associated high-temperature requirement A of Mycobacterium tuberculosis., Mohamedmohaideen NN, Palaninathan SK, Morin PM, Williams BJ, Braunstein M, Tichy SE, Locker J, Russell DH, Jacobs WR Jr, Sacchettini JC, Biochemistry. 2008 Jun 10;47(23):6092-102. Epub 2008 May 15. PMID:18479146 Page seeded by OCA on Wed Jun 11 10:46:02 2008
