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3c68
From Proteopedia
(New page: '''Unreleased structure''' The entry 3c68 is ON HOLD until Paper Publication Authors: Kurakata, Y., Uechi, A., Yoshida, H., Kamitori, S., Sakano, Y., Nishikawa, A., Tonozuka, T. Descri...) |
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| - | + | [[Image:3c68.jpg|left|200px]] | |
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| + | {{STRUCTURE_3c68| PDB=3c68 | SCENE= }} | ||
| - | + | '''Escherichia coli K12 YgjK in a complexed with mannose''' | |
| - | Description: Escherichia coli K12 YgjK in a complexed with mannose | ||
| + | ==Overview== | ||
| + | Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit. | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 | + | ==About this Structure== |
| + | 3C68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C68 OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213393 15213393] | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Single protein]] | ||
| + | [[Category: Kamitori, S.]] | ||
| + | [[Category: Kurakata, Y.]] | ||
| + | [[Category: Nishikawa, A.]] | ||
| + | [[Category: Sakano, Y.]] | ||
| + | [[Category: Tonozuka, T.]] | ||
| + | [[Category: Uechi, A.]] | ||
| + | [[Category: Yoshida, H.]] | ||
| + | [[Category: Alpha/alpha barrel]] | ||
| + | [[Category: Gh63]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Processing alpha-glucosidase]] | ||
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:48:33 2008'' | ||
Revision as of 07:48, 11 June 2008
Escherichia coli K12 YgjK in a complexed with mannose
Overview
Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit.
About this Structure
3C68 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:15213393 Page seeded by OCA on Wed Jun 11 10:48:33 2008
