2qxf
From Proteopedia
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'''Product bound structure of exonuclease I at 1.5 angstrom resolution''' | '''Product bound structure of exonuclease I at 1.5 angstrom resolution''' | ||
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| + | ==Overview== | ||
| + | In Escherichia coli, exonuclease I (ExoI) is a monomeric processive 3'-5' exonuclease that degrades single-stranded DNA. The enzyme has been implicated as primarily being involved in repairing frameshift mutations. The structure of the enzyme has previously been determined in a hexagonal space group at 2.4 A resolution. Here, the structure of ExoI in complex with a nucleotide product, thymidine 5'-monophosphate, is described in an orthorhombic space group at 1.5 A resolution. This new high-resolution structure provides some insight into the interactions involved in binding a nucleotide product. The conserved active site contains a unique metal-binding position when compared with orthologous sites in the Klenow fragment, T4 DNA polymerase and dnaQ. This unique difference is proposed to be a consequence of the repositioning of an important histidine, His181, away from the active site. | ||
==About this Structure== | ==About this Structure== | ||
2QXF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QXF OCA]. | 2QXF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QXF OCA]. | ||
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| + | ==Reference== | ||
| + | Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate., Busam RD, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):206-10. Epub 2008, Jan 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18219121 18219121] | ||
[[Category: Escherichia coli k12]] | [[Category: Escherichia coli k12]] | ||
[[Category: Exodeoxyribonuclease I]] | [[Category: Exodeoxyribonuclease I]] | ||
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[[Category: Product bound structure]] | [[Category: Product bound structure]] | ||
[[Category: Sh3-like domain]] | [[Category: Sh3-like domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:56:07 2008'' |
Revision as of 07:56, 11 June 2008
Product bound structure of exonuclease I at 1.5 angstrom resolution
Overview
In Escherichia coli, exonuclease I (ExoI) is a monomeric processive 3'-5' exonuclease that degrades single-stranded DNA. The enzyme has been implicated as primarily being involved in repairing frameshift mutations. The structure of the enzyme has previously been determined in a hexagonal space group at 2.4 A resolution. Here, the structure of ExoI in complex with a nucleotide product, thymidine 5'-monophosphate, is described in an orthorhombic space group at 1.5 A resolution. This new high-resolution structure provides some insight into the interactions involved in binding a nucleotide product. The conserved active site contains a unique metal-binding position when compared with orthologous sites in the Klenow fragment, T4 DNA polymerase and dnaQ. This unique difference is proposed to be a consequence of the repositioning of an important histidine, His181, away from the active site.
About this Structure
2QXF is a Single protein structure of sequence from Escherichia coli k12. Full crystallographic information is available from OCA.
Reference
Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate., Busam RD, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):206-10. Epub 2008, Jan 16. PMID:18219121 Page seeded by OCA on Wed Jun 11 10:56:07 2008
