3bpb
From Proteopedia
(New page: '''Unreleased structure''' The entry 3bpb is ON HOLD until Paper Publication Authors: Monzingo, A.F., Linsky, T.W., Stone, E.M., Fast, W., Robertus, J.D. Description: Crystal structure...) |
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| + | {{STRUCTURE_3bpb| PDB=3bpb | SCENE= }} | ||
| - | + | '''Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline''' | |
| - | Description: Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline | ||
| + | ==Overview== | ||
| + | Many enzymes in the pentein superfamily use a transient covalent intermediate in their catalytic mechanisms. Here we trap and determine the structure of a stable covalent adduct that mimics this intermediate using a mutant dimethylarginine dimethylaminohydrolase and an alternative substrate. The interactions observed between the enzyme and trapped adduct suggest an altered angle of attack between the nucleophiles of the first and second half-reactions of normal catalysis. The stable covalent adduct is also capable of further reaction. Addition of imidazole rescues the original hydrolytic activity. Notably, addition of other amines instead yields substituted arginine products, which arise from partitioning of the intermediate into the evolutionarily related amidinotransferase reaction pathway. The enzyme provides both selectivity and catalysis for the amidinotransferase reaction, underscoring commonalities among the reaction pathways in this mechanistically diverse enzyme superfamily. The promiscuous partitioning of this intermediate may also help to illuminate the evolutionary history of these enzymes. | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun | + | ==About this Structure== |
| + | 3BPB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BPB OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Promiscuous partitioning of a covalent intermediate common in the pentein superfamily., Linsky TW, Monzingo AF, Stone EM, Robertus JD, Fast W, Chem Biol. 2008 May;15(5):467-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18482699 18482699] | ||
| + | [[Category: Dimethylargininase]] | ||
| + | [[Category: Pseudomonas aeruginosa]] | ||
| + | [[Category: Single protein]] | ||
| + | [[Category: Fast, W.]] | ||
| + | [[Category: Linsky, T W.]] | ||
| + | [[Category: Monzingo, A F.]] | ||
| + | [[Category: Robertus, J D.]] | ||
| + | [[Category: Stone, E M.]] | ||
| + | [[Category: Enzyme adduct]] | ||
| + | [[Category: Hydrolase]] | ||
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:08:16 2008'' | ||
Revision as of 09:08, 18 June 2008
Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline
Overview
Many enzymes in the pentein superfamily use a transient covalent intermediate in their catalytic mechanisms. Here we trap and determine the structure of a stable covalent adduct that mimics this intermediate using a mutant dimethylarginine dimethylaminohydrolase and an alternative substrate. The interactions observed between the enzyme and trapped adduct suggest an altered angle of attack between the nucleophiles of the first and second half-reactions of normal catalysis. The stable covalent adduct is also capable of further reaction. Addition of imidazole rescues the original hydrolytic activity. Notably, addition of other amines instead yields substituted arginine products, which arise from partitioning of the intermediate into the evolutionarily related amidinotransferase reaction pathway. The enzyme provides both selectivity and catalysis for the amidinotransferase reaction, underscoring commonalities among the reaction pathways in this mechanistically diverse enzyme superfamily. The promiscuous partitioning of this intermediate may also help to illuminate the evolutionary history of these enzymes.
About this Structure
3BPB is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Promiscuous partitioning of a covalent intermediate common in the pentein superfamily., Linsky TW, Monzingo AF, Stone EM, Robertus JD, Fast W, Chem Biol. 2008 May;15(5):467-75. PMID:18482699 Page seeded by OCA on Wed Jun 18 12:08:16 2008
