3d91
From Proteopedia
(New page: '''Unreleased structure''' The entry 3d91 is ON HOLD Authors: Prade, L., Bezencon, O., Bur, D., Weller, T., Fischli, W., Remen, L. Description: Human renin in complex with remikiren ...) |
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| - | + | [[Image:3d91.jpg|left|200px]] | |
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| + | {{STRUCTURE_3d91| PDB=3d91 | SCENE= }} | ||
| - | + | '''Human renin in complex with remikiren''' | |
| - | Description: Human renin in complex with remikiren | ||
| + | ==Overview== | ||
| + | Human renin has been expressed in Sf9 and CHO cells using two different gene constructs. The first construct contained a foreign signal peptide fused directly to the sequence encoding mature renin, whereas the second construct harbors the sequence for preprorenin. Prorenin was produced in significantly higher amounts than the mature enzyme expressed without its propeptide in both expression systems. Both directly expressed mature renin and proteolytically derived active renin have been purified and cocrystallized with the renin inhibitor Ro 42-5892. The 3D structure has been solved for both versions and demonstrates identity despite different glycosylation and different N termini. | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun | + | ==Disease== |
| + | Known disease associated with this structure: Renal tubular dysgenesis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=179820 179820]], Hyperproreninemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=179820 179820]] | ||
| + | |||
| + | ==About this Structure== | ||
| + | 3D91 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D91 OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Recombinant human renin produced in different expression systems: biochemical properties and 3D structure., Mathews S, Dobeli H, Pruschy M, Bosser R, D'Arcy A, Oefner C, Zulauf M, Gentz R, Breu V, Matile H, Schlaeger J, Fischli W, Protein Expr Purif. 1996 Feb;7(1):81-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9172787 9172787] | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Renin]] | ||
| + | [[Category: Single protein]] | ||
| + | [[Category: Bezencon, O.]] | ||
| + | [[Category: Bur, D.]] | ||
| + | [[Category: Fischli, W.]] | ||
| + | [[Category: Prade, L.]] | ||
| + | [[Category: Remen, L.]] | ||
| + | [[Category: Weller, T.]] | ||
| + | [[Category: Alternative splicing]] | ||
| + | [[Category: Aspartyl protease]] | ||
| + | [[Category: Cleavage on pair of basic residue]] | ||
| + | [[Category: Disease mutation]] | ||
| + | [[Category: Glycoprotein]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Membrane]] | ||
| + | [[Category: Polymorphism]] | ||
| + | [[Category: Protease]] | ||
| + | [[Category: Remikiren]] | ||
| + | [[Category: Renin]] | ||
| + | [[Category: Secreted]] | ||
| + | [[Category: Zymogen]] | ||
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:13:09 2008'' | ||
Revision as of 09:13, 18 June 2008
Human renin in complex with remikiren
Contents |
Overview
Human renin has been expressed in Sf9 and CHO cells using two different gene constructs. The first construct contained a foreign signal peptide fused directly to the sequence encoding mature renin, whereas the second construct harbors the sequence for preprorenin. Prorenin was produced in significantly higher amounts than the mature enzyme expressed without its propeptide in both expression systems. Both directly expressed mature renin and proteolytically derived active renin have been purified and cocrystallized with the renin inhibitor Ro 42-5892. The 3D structure has been solved for both versions and demonstrates identity despite different glycosylation and different N termini.
Disease
Known disease associated with this structure: Renal tubular dysgenesis OMIM:[179820], Hyperproreninemia OMIM:[179820]
About this Structure
3D91 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Recombinant human renin produced in different expression systems: biochemical properties and 3D structure., Mathews S, Dobeli H, Pruschy M, Bosser R, D'Arcy A, Oefner C, Zulauf M, Gentz R, Breu V, Matile H, Schlaeger J, Fischli W, Protein Expr Purif. 1996 Feb;7(1):81-91. PMID:9172787 Page seeded by OCA on Wed Jun 18 12:13:09 2008
Categories: Homo sapiens | Renin | Single protein | Bezencon, O. | Bur, D. | Fischli, W. | Prade, L. | Remen, L. | Weller, T. | Alternative splicing | Aspartyl protease | Cleavage on pair of basic residue | Disease mutation | Glycoprotein | Hydrolase | Membrane | Polymorphism | Protease | Remikiren | Secreted | Zymogen
