1m5k
From Proteopedia
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{{STRUCTURE_1m5k| PDB=1m5k | SCENE= }} | {{STRUCTURE_1m5k| PDB=1m5k | SCENE= }} | ||
| - | ''' | + | '''Crystal structure of a hairpin ribozyme in the catalytically-active conformation''' |
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==About this Structure== | ==About this Structure== | ||
| - | 1M5K is a [[ | + | 1M5K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hp6 1hp6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5K OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis., Rupert PB, Ferre-D'Amare AR, Nature. 2001 Apr 12;410(6830):780-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11298439 11298439] | Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis., Rupert PB, Ferre-D'Amare AR, Nature. 2001 Apr 12;410(6830):780-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11298439 11298439] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: | + | [[Category: Protein complex]] |
[[Category: Amare, A R.Ferre-D.]] | [[Category: Amare, A R.Ferre-D.]] | ||
[[Category: Rupert, P B.]] | [[Category: Rupert, P B.]] | ||
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[[Category: Hairpin ribozyme]] | [[Category: Hairpin ribozyme]] | ||
[[Category: Substrate inhibitor strand]] | [[Category: Substrate inhibitor strand]] | ||
| + | [[Category: Translation/rna complex]] | ||
[[Category: U1a rna binding protein docked conformation]] | [[Category: U1a rna binding protein docked conformation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:13:57 2008'' |
Revision as of 09:14, 18 June 2008
Crystal structure of a hairpin ribozyme in the catalytically-active conformation
Overview
The hairpin ribozyme catalyses sequence-specific cleavage of RNA. The active site of this natural RNA results from the docking of two irregular helices: stems A and B. One strand of stem A harbours the scissile bond. The 2.4 A resolution structure of a hairpin ribozyme-inhibitor complex reveals that the ribozyme aligns the 2'-OH nucleophile and the 5'-oxo leaving group by twisting apart the nucleotides that flank the scissile phosphate. The base of the nucleotide preceding the cleavage site is stacked within stem A; the next nucleotide, a conserved guanine, is extruded from stem A and accommodated by a highly complementary pocket in the minor groove of stem B. Metal ions are absent from the active site. The bases of four conserved purines are positioned potentially to serve as acid-base catalysts. This is the first structure determination of a fully assembled ribozyme active site that catalyses a phosphodiester cleavage without recourse to metal ions.
About this Structure
1M5K is a Protein complex structure of sequences from Homo sapiens. This structure supersedes the now removed PDB entry 1hp6. Full crystallographic information is available from OCA.
Reference
Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis., Rupert PB, Ferre-D'Amare AR, Nature. 2001 Apr 12;410(6830):780-6. PMID:11298439 Page seeded by OCA on Wed Jun 18 12:13:57 2008
