2o4f
From Proteopedia
| Line 11: | Line 11: | ||
'''Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions''' | '''Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions''' | ||
| + | |||
| + | ==Overview== | ||
| + | A parallel 5'-d(TGGGGT)-3' quadruplex was formed in Na(+) solution and crystallized using lithium sulfate as the main precipitating agent. The X-ray structure was determined to 1.5 A resolution in space group P2(1) by molecular replacement. The asymmetric unit consists of a characteristic motif of two quadruplexes stacked at their 5' ends. All nucleotides are clearly defined in the density and could be positioned. A single bound Li(+) ion is observed at the surface of the column formed by the two joined molecules. Thus, this small alkali metal ion appears to be unsuitable as a replacement for the Na(+) ion in the central channel of G-quartets, unlike K(+) or Tl(+) ions. A well conserved constellation of water molecules is observed in the grooves of the dimeric structure. | ||
==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O4F OCA]. | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O4F OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Structure of a d(TGGGGT) quadruplex crystallized in the presence of Li+ ions., Creze C, Rinaldi B, Haser R, Bouvet P, Gouet P, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):682-8. Epub 2007, May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17505106 17505106] | ||
[[Category: Bouvet, P.]] | [[Category: Bouvet, P.]] | ||
[[Category: Creze, C.]] | [[Category: Creze, C.]] | ||
| Line 22: | Line 28: | ||
[[Category: Parallel-stranded]] | [[Category: Parallel-stranded]] | ||
[[Category: Quadruple helix]] | [[Category: Quadruple helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:16:20 2008'' |
Revision as of 09:16, 18 June 2008
Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions
Overview
A parallel 5'-d(TGGGGT)-3' quadruplex was formed in Na(+) solution and crystallized using lithium sulfate as the main precipitating agent. The X-ray structure was determined to 1.5 A resolution in space group P2(1) by molecular replacement. The asymmetric unit consists of a characteristic motif of two quadruplexes stacked at their 5' ends. All nucleotides are clearly defined in the density and could be positioned. A single bound Li(+) ion is observed at the surface of the column formed by the two joined molecules. Thus, this small alkali metal ion appears to be unsuitable as a replacement for the Na(+) ion in the central channel of G-quartets, unlike K(+) or Tl(+) ions. A well conserved constellation of water molecules is observed in the grooves of the dimeric structure.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of a d(TGGGGT) quadruplex crystallized in the presence of Li+ ions., Creze C, Rinaldi B, Haser R, Bouvet P, Gouet P, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):682-8. Epub 2007, May 15. PMID:17505106 Page seeded by OCA on Wed Jun 18 12:16:20 2008
