2ro1
From Proteopedia
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'''NMR Solution Structures of Human KAP1 PHD finger-bromodomain''' | '''NMR Solution Structures of Human KAP1 PHD finger-bromodomain''' | ||
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| + | ==Overview== | ||
| + | The tandem PHD finger-bromodomain, found in many chromatin-associated proteins, has an important role in gene silencing by the human co-repressor KRAB-associated protein 1 (KAP1). Here we report the three-dimensional solution structure of the tandem PHD finger-bromodomain of KAP1. The structure reveals a distinct scaffold unifying the two protein modules, in which the first helix, alpha(Z), of an atypical bromodomain forms the central hydrophobic core that anchors the other three helices of the bromodomain on one side and the zinc binding PHD finger on the other. A comprehensive mutation-based structure-function analysis correlating transcriptional repression, ubiquitin-conjugating enzyme 9 (UBC9) binding and SUMOylation shows that the PHD finger and the bromodomain of KAP1 cooperate as one functional unit to facilitate lysine SUMOylation, which is required for KAP1 co-repressor activity in gene silencing. These results demonstrate a previously unknown unified function for the tandem PHD finger-bromodomain as an intramolecular small ubiquitin-like modifier (SUMO) E3 ligase for transcriptional silencing. | ||
==About this Structure== | ==About this Structure== | ||
2RO1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO1 OCA]. | 2RO1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO1 OCA]. | ||
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| + | ==Reference== | ||
| + | Structural insights into human KAP1 PHD finger-bromodomain and its role in gene silencing., Zeng L, Yap KL, Ivanov AV, Wang X, Mujtaba S, Plotnikova O, Rauscher FJ 3rd, Zhou MM, Nat Struct Mol Biol. 2008 Jun;15(6):626-33. Epub 2008 May 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18488044 18488044] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Zinc]] | [[Category: Zinc]] | ||
[[Category: Zinc-finger]] | [[Category: Zinc-finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:17:10 2008'' |
Revision as of 09:17, 18 June 2008
NMR Solution Structures of Human KAP1 PHD finger-bromodomain
Overview
The tandem PHD finger-bromodomain, found in many chromatin-associated proteins, has an important role in gene silencing by the human co-repressor KRAB-associated protein 1 (KAP1). Here we report the three-dimensional solution structure of the tandem PHD finger-bromodomain of KAP1. The structure reveals a distinct scaffold unifying the two protein modules, in which the first helix, alpha(Z), of an atypical bromodomain forms the central hydrophobic core that anchors the other three helices of the bromodomain on one side and the zinc binding PHD finger on the other. A comprehensive mutation-based structure-function analysis correlating transcriptional repression, ubiquitin-conjugating enzyme 9 (UBC9) binding and SUMOylation shows that the PHD finger and the bromodomain of KAP1 cooperate as one functional unit to facilitate lysine SUMOylation, which is required for KAP1 co-repressor activity in gene silencing. These results demonstrate a previously unknown unified function for the tandem PHD finger-bromodomain as an intramolecular small ubiquitin-like modifier (SUMO) E3 ligase for transcriptional silencing.
About this Structure
2RO1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural insights into human KAP1 PHD finger-bromodomain and its role in gene silencing., Zeng L, Yap KL, Ivanov AV, Wang X, Mujtaba S, Plotnikova O, Rauscher FJ 3rd, Zhou MM, Nat Struct Mol Biol. 2008 Jun;15(6):626-33. Epub 2008 May 18. PMID:18488044 Page seeded by OCA on Wed Jun 18 12:17:10 2008
Categories: Homo sapiens | Single protein | Ivanov, A V. | Mujtaba, S. | Plotnikova, O. | Rauscher, F J. | Wang, X. | Yap, K L. | Zeng, L. | Acetylation | Alternative splicing | Bromodomain | Kap | Metal-binding | Nucleus | Phd finger | Phosphoprotein | Repressor | Sumo | Tif | Transcription | Transcription regulation | Zinc | Zinc-finger
