1cej
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(New page: 200px<br /><applet load="1cej" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cej" /> '''SOLUTION STRUCTURE OF AN EGF MODULE PAIR FRO...)
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Revision as of 10:16, 20 November 2007
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SOLUTION STRUCTURE OF AN EGF MODULE PAIR FROM THE PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1
Overview
The solution structure of the 96-residue C-terminal fragment of the, merozoite surface protein 1 (MSP-1) from Plasmodium falciparum has been, determined using nuclear magnetic resonance (NMR) spectroscopic, measurements on uniformly13C/15N-labelled protein, efficiently expressed, in the methylotrophic yeast Komagataella (Pichia) pastoris. The structure, has two domains with epidermal growth factor (EGF)-like folds with a novel, domain interface for the EGF domain pair interactions, formed from a, cluster of hydrophobic residues. This gives the protein a U-shaped overall, structure with the N-terminal proteolytic processing site close to the, C-terminal glycosyl phosphatidyl inositol (GPI) membrane anchor site, which is consistent with the involvement of a membrane-bound proteinase in, the processing of MSP-1 during erythrocyte invasion. This structure, which, is the first protozoan EGF example to be determined, contrasts with the, elongated structures seen for EGF-module pairs having shared Ca2+-ligation, sites at their interface, as found, for example, in fibrillin-1., Recognition surfaces for antibodies that inhibit processing and invasion, and antibodies that block the binding of these inhibitory antibodies, have, been mapped on the three-dimensional structure by considering specific, MSP-1 mutants.
About this Structure
1CEJ is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1., Morgan WD, Birdsall B, Frenkiel TA, Gradwell MG, Burghaus PA, Syed SE, Uthaipibull C, Holder AA, Feeney J, J Mol Biol. 1999 May 28;289(1):113-22. PMID:10339410
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