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1cgt
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(New page: 200px<br /><applet load="1cgt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cgt, resolution 2.0Å" /> '''STRUCTURE OF CYCLODEX...)
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Revision as of 10:19, 20 November 2007
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STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION
Overview
The previously reported structural model of cyclodextrin, glycosyltransferase (EC 2.4.1.19) from Bacillus circulans has been, improved. For this purpose the known sequence was built into an electron, density map established by multiple isomorphous replacement and subsequent, solvent-flattening at 2.5 A resolution. The resulting model was refined at, 2.0 A resolution using a simulated annealing refinement method. Based on, 70,171 independent reflections in the range 7.0 to 2.0 A resolution, a, final R-factor of 17.6% was obtained with a model obeying standard, geometry within 0.013 A in bond lengths and 2.7 degrees in bond angles., The final model consists of all 684 amino acid residues, two calcium ions, and 588 solvent molecules.
About this Structure
1CGT is a Single protein structure of sequence from Bacillus circulans with CA as ligand. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.
Reference
Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution., Klein C, Schulz GE, J Mol Biol. 1991 Feb 20;217(4):737-50. PMID:1826034
Page seeded by OCA on Tue Nov 20 12:26:14 2007
