1cxc
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(New page: 200px<br /><applet load="1cxc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cxc, resolution 1.6Å" /> '''CRYSTALLIZATION AND X...)
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Revision as of 10:42, 20 November 2007
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CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS
Overview
Cytochrome c(2) serves as the secondary electron donor that reduces the, photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria., Cytochrome c(2) from Rhodobacter sphaeroides has been crystallized in, three different forms. At high ionic strength, crystals of a hexagonal, space group (P6(1)22) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4(1)2(1)2) crystals were formed. The, three-dimensional structures of the cytochrome in all three crystal forms, have been determined by X-ray diffraction at resolutions of 2.20 A, (hexagonal), 1.95 A, (triclinic) and 1.53 A (tetragonal). The most, significant difference observed was the binding of an imidazole molecule, to the iron atom of the heme group in the hexagonal structure. This, binding displaces the sulfur atom of Met l00, which forms the axial ligand, in the triclinic and tetragonal structures.
About this Structure
1CXC is a Single protein structure of sequence from Rhodobacter sphaeroides with HEM as ligand. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray structure determination of cytochrome c2 from Rhodobacter sphaeroides in three crystal forms., Axelrod HL, Feher G, Allen JP, Chirino AJ, Day MW, Hsu BT, Rees DC, Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):596-602. PMID:15299423
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