1cxl
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(New page: 200px<br /><applet load="1cxl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cxl, resolution 1.810Å" /> '''COMPLEX BETWEEN A C...)
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Revision as of 10:42, 20 November 2007
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COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q
Overview
Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the, alpha-amylase family, which uses a double displacement mechanism to, process alpha-linked glucose polymers. We have determined two X-ray, structures of CGTase complexes, one with an intact substrate at 2.1 A, resolution, and the other with a covalently bound reaction intermediate at, 1.8 A resolution. These structures give evidence for substrate distortion, and the covalent character of the intermediate and for the first time, show, in atomic detail, how catalysis in the alpha-amylase family proceeds, by the concerted action of all active site residues.
About this Structure
1CXL is a Single protein structure of sequence from Bacillus circulans with CA and MPD as ligands. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.
Reference
X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family., Uitdehaag JC, Mosi R, Kalk KH, van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW, Nat Struct Biol. 1999 May;6(5):432-6. PMID:10331869
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