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1bs0
From Proteopedia
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(New page: 200px<br /> <applet load="1bs0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bs0, resolution 1.65Å" /> '''PLP-DEPENDENT ACYL-...)
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Revision as of 17:30, 29 October 2007
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PLP-DEPENDENT ACYL-COA SYNTHASE
Overview
8-Amino-7-oxononanoate synthase (or 8-amino-7-ketopelargonate synthase; EC, 2.3.1.47; AONS) catalyses the decarboxylative condensation of l-alanine, and pimeloyl-CoA in the first committed step of biotin biosynthesis. We, have cloned, over-expressed and purified AONS from Escherichia coli and, determined the crystal structures of the apo and PLP-bound forms of the, enzyme. The protein is a symmetrical homodimer with a tertiary structure, and active site organisation similar to, but distinct from, those of other, PLP-dependent enzymes whose three-dimensional structures are known. The, critical PLP-binding lysine of AONS is located at the end of a deep cleft, that allows access of the pantothenate arm of pimeloyl-CoA. A cluster of, positively charged residues at the entrance to this cleft ... [(full description)]
About this Structure
1BS0 is a [Single protein] structure of sequence from [Escherichia coli] with SO4 as [ligand]. Active as [[1]], with EC number [2.3.1.47]. Full crystallographic information is available from [OCA].
Reference
The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme., Alexeev D, Alexeeva M, Baxter RL, Campopiano DJ, Webster SP, Sawyer L, J Mol Biol. 1998 Nov 27;284(2):401-19. PMID:9813126
Page seeded by OCA on Mon Oct 29 19:34:46 2007
