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1d2p
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(New page: 200px<br /><applet load="1d2p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d2p, resolution 2.50Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 10:51, 20 November 2007
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CRYSTAL STRUCTURE OF TWO B REPEAT UNITS (B1B2) OF THE COLLAGEN BINDING PROTEIN (CNA) OF STAPHYLOCOCCUS AUREUS
Overview
BACKGORUND: The Staphylococcus aureus collagen-binding protein Cna, mediates bacterial adherence to collagen. The primary sequence of Cna has, a non-repetitive collagen-binding A region, followed by the repetitive B, region. The B region has one to four 23 kDa repeat units (B(1)-B(4)), depending on the strain of origin. The affinity of the A region for, collagen is independent of the B region. However, the B repeat units have, been suggested to serve as a 'stalk' that projects the A region from the, bacterial surface and thus facilitate bacterial adherence to collagen. To, understand the biological role of these B-region repeats we determined, their three-dimensional structure. RESULTS: B(1) has two domains (D(1) and, D(2)) placed side-by-side. D(1) and D(2) have similar secondary structure, and exhibit a unique fold that resembles but is the inverse of the, immunoglobulin-like (IgG-like) domains. Comparison with similar, immunoglobulin superfamily (IgSF) structures shows novel packing, arrangements between the D(1) and D(2) domains. In the B(1)B(2) crystal, structure, an omission of a single glycine residue in the D(2)-D(3) linker, loop, compared to the D(1)-D(2) and D(3)-D(4) linker loops, resulted in, projection of the D(3) and D(4) in a spatially new orientation. We also, present a model for B(1)B(2)B(3)B(4). CONCLUSIONS: The B region of the Cna, collagen adhesin has a novel fold that is reminiscent of but is inverse in, nature to the IgG fold. This B region assembly could effectively provide, the needed flexibility and stability for presenting the ligand binding A, region away from the bacterial cell surface.
About this Structure
1D2P is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Novel fold and assembly of the repetitive B region of the Staphylococcus aureus collagen-binding surface protein., Deivanayagam CC, Rich RL, Carson M, Owens RT, Danthuluri S, Bice T, Hook M, Narayana SV, Structure. 2000 Jan 15;8(1):67-78. PMID:10673425
Page seeded by OCA on Tue Nov 20 12:58:25 2007
