1d8i

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Revision as of 10:57, 20 November 2007

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spinqualitylabelsall models 1d8i, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH A SULFATE ION.

Overview

RNA triphosphatase is an essential mRNA processing enzyme that catalyzes, the first step in cap formation. The 2.05 A crystal structure of yeast RNA, triphosphatase Cet1p reveals a novel active site fold whereby an, eight-stranded beta barrel forms a topologically closed triphosphate, tunnel. Interactions of a sulfate in the center of the tunnel with a, divalent cation and basic amino acids projecting into the tunnel suggest a, catalytic mechanism that is supported by mutational data. Discrete surface, domains mediate Cet1p homodimerization and Cet1p binding to the, guanylyltransferase component of the capping apparatus. The structure and, mechanism of fungal RNA triphosphatases are completely different from, those of mammalian mRNA capping enzymes. Hence, RNA triphosphatase, presents an ideal target for structure-based antifungal drug discovery.

About this Structure

1D8I is a Single protein structure of sequence from Saccharomyces cerevisiae with SO4 as ligand. Active as Polynucleotide 5'-phosphatase, with EC number 3.1.3.33 Full crystallographic information is available from OCA.

Reference

Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus., Lima CD, Wang LK, Shuman S, Cell. 1999 Nov 24;99(5):533-43. PMID:10589681

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