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1k9o

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==Overview==
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{{ABSTRACT_11685246}}
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Serine protease inhibitors (serpins) regulate the activities of circulating proteases. Serpins inhibit proteases by acylating the serine hydroxyl at their active sites. Before deacylation and complete proteolysis of the serpin can occur, massive conformational changes are triggered in the serpin while maintaining the covalent linkage between the protease and serpin. Here we report the structure of a serpin-trypsin Michaelis complex, which we visualized by using the S195A trypsin mutant to prevent covalent complex formation. This encounter complex reveals a more extensive interaction surface than that present in small inhibitor-protease complexes and is a template for modeling other serpin-protease pairs. Mutations of several serpin residues at the interface reduced the inhibitory activity of the serpin. The serine residue C-terminal to the scissile peptide bond is found in a closer than usual interaction with His 57 at the active site of trypsin.
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==About this Structure==
==About this Structure==
1K9O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1i99 1i99]. The following page contains interesting information on the relation of 1K9O with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb53_1.html Serpins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9O OCA].
1K9O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1i99 1i99]. The following page contains interesting information on the relation of 1K9O with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb53_1.html Serpins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9O OCA].
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==Reference==
 
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The structure of a Michaelis serpin-protease complex., Ye S, Cech AL, Belmares R, Bergstrom RC, Tong Y, Corey DR, Kanost MR, Goldsmith EJ, Nat Struct Biol. 2001 Nov;8(11):979-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11685246 11685246]
 
[[Category: Manduca sexta]]
[[Category: Manduca sexta]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Ye, S.]]
[[Category: Ye, S.]]
[[Category: Michaelis serpin-protease complex inhibitory triad]]
[[Category: Michaelis serpin-protease complex inhibitory triad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:28:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 19:18:20 2008''

Revision as of 16:18, 25 June 2008

Image:1k9o.png

Template:STRUCTURE 1k9o

CRYSTAL STRUCTURE OF MICHAELIS SERPIN-TRYPSIN COMPLEX


Template:ABSTRACT 11685246

About this Structure

1K9O is a Protein complex structure of sequences from Manduca sexta and Rattus norvegicus. This structure supersedes the now removed PDB entry 1i99. The following page contains interesting information on the relation of 1K9O with [Serpins]. Full crystallographic information is available from OCA.

Page seeded by OCA on Wed Jun 25 19:18:20 2008

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