1dv0

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==Overview==
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{{ABSTRACT_11087358}}
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The DNA repair protein HHR23A is a highly conserved protein that functions in nucleotide excision repair. HHR23A contains two ubiquitin associated domains (UBA) that are conserved in a number of proteins with diverse functions involved in ubiquitination, UV excision repair, and signaling pathways via protein kinases. The cellular binding partners of UBA domains remain unclear; however, we previously found that the HHR23A UBA(2) domain interacts specifically with the HIV-1 Vpr protein. Analysis of the low resolution solution structure of HHR23A UBA(2) revealed a hydrophobic loop region of the UBA(2) domain that we predicted was the interface for protein/protein interactions. Here we present results of in vitro binding studies that demonstrate the requirement of this hydrophobic loop region for interaction with human immunodeficiency virus (HIV-1) Vpr. A single point mutation of the Pro at residue 333 to a Glu totally abolishes the binding of HIV-1 Vpr to UBA(2). High resolution NMR structures of the binding deficient UBA(2) mutant P333E as well as of the wild-type UBA(2) domain were determined to compare the effect of this mutation on the structure. Small but significant differences are observed only locally at the site of the mutation. The biochemical and structural analysis confirms the function of the HHR23A UBA(2) GFP-loop as the protein/protein interacting domain.
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==About this Structure==
==About this Structure==
1DV0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1uba 1uba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV0 OCA].
1DV0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1uba 1uba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV0 OCA].
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==Reference==
 
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Biochemical and structural analysis of the interaction between the UBA(2) domain of the DNA repair protein HHR23A and HIV-1 Vpr., Withers-Ward ES, Mueller TD, Chen IS, Feigon J, Biochemistry. 2000 Nov 21;39(46):14103-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11087358 11087358]
 
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Withers-Ward, E S.]]
[[Category: Withers-Ward, E S.]]
[[Category: Helical bundle]]
[[Category: Helical bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:18:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 19:21:49 2008''

Revision as of 16:21, 25 June 2008

Image:1dv0.png

Template:STRUCTURE 1dv0

Refined NMR solution structure of the C-terminal UBA domain of the human homologue of RAD23A (HHR23A)


Template:ABSTRACT 11087358

About this Structure

1DV0 is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1uba. Full crystallographic information is available from OCA.

Page seeded by OCA on Wed Jun 25 19:21:49 2008

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