1ana

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[[Image:1ana.jpg|left|200px]]
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==Overview==
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{{ABSTRACT_6726797}}
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The DNA oligomer of sequence IC-C-G-G has been synthesized, and its X-ray crystal structure solved at a resolution of 2.0 A, using anomalous scattering from iodines in phase analysis: 48 cycles of Jack-Levitt restrained least-squares refinement resulted in a residual error of 19.9% over all data, or 16.5% for two-sigma data. Two double-helical tetramers stack in the crystal to form a continuous octamer, except for the two missing phosphate connections across the center. The octamer has a mean helix rotation of 33.7 degrees (10.7 base-pairs per turn), rise of 2.87 A, mean inclination angle of base-pairs of 14 degrees, and mean base-pair propeller twist of +16.3 degrees. Local variations in both helix rotation and base plane roll angles, including those across the center of the octamer, are as predicted from base sequence by sum functions sigma 1 and sigma 2. The three known DNA octamers: IC-C-G-G/IC-C-G-G, G-G-T-A-T-A-C-C and G-G-C-C-G-G-C-C, make up a graded series in this order, with monotonically changing structural parameters. An exhaustive comparison of torsion angle correlations among the known A helices confirms some structural expectations and reveals some new features. 86 water molecules have been located per double-helical IC-C-G-G tetramer (the asymmetric unit), of which 451/2 per tetramer lie within a first hydrogen-bonded shell of hydration. No ordered water structure is observed comparable to the minor groove spine of hydration in B-DNA.
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==About this Structure==
==About this Structure==
The following page contains interesting information on the relation of 1ANA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb23_1.html DNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ANA OCA].
The following page contains interesting information on the relation of 1ANA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb23_1.html DNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ANA OCA].
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==Reference==
 
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Helix geometry and hydration in an A-DNA tetramer: IC-C-G-G., Conner BN, Yoon C, Dickerson JL, Dickerson RE, J Mol Biol. 1984 Apr 25;174(4):663-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6726797 6726797]
 
[[Category: DNA]]
[[Category: DNA]]
[[Category: Conner, B N.]]
[[Category: Conner, B N.]]
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[[Category: Double helix]]
[[Category: Double helix]]
[[Category: Modified]]
[[Category: Modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:28:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:03:08 2008''

Revision as of 14:03, 26 June 2008

Image:1ana.png

Template:STRUCTURE 1ana

HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G


Template:ABSTRACT 6726797

About this Structure

The following page contains interesting information on the relation of 1ANA with [DNA]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:03:08 2008

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