From Proteopedia
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| - | ==Overview==
| + | {{ABSTRACT_9285585}} |
| - | Chaperonins assist protein folding with the consumption of ATP. They exist as multi-subunit protein assemblies comprising rings of subunits stacked back to back. In Escherichia coli, asymmetric intermediates of GroEL are formed with the co-chaperonin GroES and nucleotides bound only to one of the seven-subunit rings (the cis ring) and not to the opposing ring (the trans ring). The structure of the GroEL-GroES-(ADP)7 complex reveals how large en bloc movements of the cis ring's intermediate and apical domains enable bound GroES to stabilize a folding chamber with ADP confined to the cis ring. Elevation and twist of the apical domains double the volume of the central cavity and bury hydrophobic peptide-binding residues in the interface with GroES, as well as between GroEL subunits, leaving a hydrophilic cavity lining that is conducive to protein folding. An inward tilt of the cis equatorial domain causes an outward tilt in the trans ring that opposes the binding of a second GroES. When combined with new functional results, this negative allosteric mechanism suggests a model for an ATP-driven folding cycle that requires a double toroid.
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| | ==About this Structure== | | ==About this Structure== |
| | 1AON is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1AON with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb32_1.html Chaperones]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AON OCA]. | | 1AON is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1AON with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb32_1.html Chaperones]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AON OCA]. |
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| - | ==Reference== | |
| - | The crystal structure of the asymmetric GroEL-GroES-(ADP)7 chaperonin complex., Xu Z, Horwich AL, Sigler PB, Nature. 1997 Aug 21;388(6644):741-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9285585 9285585] | |
| | [[Category: Chaperones]] | | [[Category: Chaperones]] |
| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
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| | [[Category: Xu, Z.]] | | [[Category: Xu, Z.]] |
| | [[Category: Chaperonin assisted protein folding]] | | [[Category: Chaperonin assisted protein folding]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:31:20 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:03:29 2008'' |
Revision as of 14:03, 26 June 2008
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| 1aon, resolution 3.00Å ()
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| Ligands:
| ,
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| Gene:
| GROE (Escherichia coli)
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| Structural annotation:
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| Resources:
| CATH : 1Aona01, 1Aona03, 1Aona02, 1Aonb03, 1Aonb01, 1Aonb02, 1Aonc02, 1Aonc01, 1Aonc03, 1Aond01, 1Aond02, 1Aond03, 1Aone03, 1Aone01, 1Aone02, 1Aonf01, 1Aonf02, 1Aonf03, 1Aong02, 1Aong03, 1Aong01, 1Aonh02, 1Aonh03, 1Aonh01, 1Aoni03, 1Aoni01, 1Aoni02, 1Aonj03, 1Aonj02, 1Aonj01, 1Aonk02, 1Aonk01, 1Aonk03, 1Aonl03, 1Aonl02, 1Aonl01, 1Aonm02, 1Aonm01, 1Aonm03, 1Aonn02, 1Aonn01, 1Aonn03, 1Aono00, 1Aonp00, 1Aonq00, 1Aonr00, 1Aons00, 1Aont00, 1Aonu00
InterPro : Ipr002423, Ipr001844, Ipr008950, Ipr012723, Ipr011032, Ipr001476
Pfam : PF00118, PF00166
SCOP : d1aona1, d1aona2, d1aona3, d1aonb1, d1aonb3, d1aonb2, d1aonc2, d1aonc3, d1aonc1, d1aond1, d1aond2, d1aond3, d1aone2, d1aone1, d1aone3, d1aonf2, d1aonf1, d1aonf3, d1aong3, d1aong2, d1aong1, d1aonh1, d1aonh2, d1aonh3, d1aoni2, d1aoni3, d1aoni1, d1aonj1, d1aonj3, d1aonj2, d1aonk1, d1aonk3, d1aonk2, d1aonl2, d1aonl3, d1aonl1, d1aonm1, d1aonm3, d1aonm2, d1aonn3, d1aonn1, d1aonn2, d1aono_, d1aonp_, d1aonq_, d1aonr_, d1aons_, d1aont_, d1aonu_
UniProt : P0A6F5, P0A6F9
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| Resources:
| FirstGlance, OCA, PDBsum, RCSB
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| Coordinates:
| save as pdb, mmCIF, xml
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CRYSTAL STRUCTURE OF THE ASYMMETRIC CHAPERONIN COMPLEX GROEL/GROES/(ADP)7
Template:ABSTRACT 9285585
About this Structure
1AON is a Protein complex structure of sequences from Escherichia coli. The following page contains interesting information on the relation of 1AON with [Chaperones]. Full crystallographic information is available from OCA.
Page seeded by OCA on Thu Jun 26 17:03:29 2008
