1deq
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(New page: 200px<br /><applet load="1deq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1deq, resolution 3.5Å" /> '''THE CRYSTAL STRUCTURE...)
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Revision as of 11:06, 20 November 2007
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THE CRYSTAL STRUCTURE OF MODIFIED BOVINE FIBRINOGEN (AT ~4 ANGSTROM RESOLUTION)
Overview
Here we report the crystal structure at approximately 4-A resolution of a, selectively proteolyzed bovine fibrinogen. This key component in, hemostasis is an elongated 340-kDa glycoprotein in the plasma that upon, activation by thrombin self-assembles to form the fibrin clot. The, crystals are unusual because they are made up of end-to-end bonded, molecules that form flexible filaments. We have visualized the entire, coiled-coil region of the molecule, which has a planar sigmoidal shape., The primary polymerization receptor pockets at the ends of the molecule, face the same way throughout the end-to-end bonded filaments, and based on, this conformation, we have developed an improved model of the two-stranded, protofibril that is the basic building block in fibrin. Near the middle of, the coiled-coil region, the plasmin-sensitive segment is a hinge about, which the molecule adopts different conformations. This segment also, includes the boundary between the three- and four-stranded portions of the, coiled coil, indicating the location on the backbone that anchors the, extended flexible Aalpha arm. We suggest that a flexible branch point in, the molecule may help accommodate variability in the structure of the, fibrin clot.
About this Structure
1DEQ is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.
Reference
The crystal structure of modified bovine fibrinogen., Brown JH, Volkmann N, Jun G, Henschen-Edman AH, Cohen C, Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):85-90. PMID:10618375
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