1eqj

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[[Image:1eqj.gif|left|200px]]
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==Overview==
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{{ABSTRACT_10764795}}
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The structure of the complex between the 2, 3-diphosphoglycerate-independent phosphoglycerate mutase (iPGM) from Bacillus stearothermophilus and its 3-phosphoglycerate substrate has recently been solved, and analysis of this structure allowed formulation of a mechanism for iPGM catalysis. In order to obtain further evidence for this mechanism, we have solved the structure of this iPGM complexed with 2-phosphoglycerate and two Mn(2+) ions at 1. 7-A resolution. The structure consists of two different domains connected by two loops and interacting through a network of hydrogen bonds. This structure is consistent with the proposed mechanism for iPGM catalysis, with the two main steps in catalysis being a phosphatase reaction removing the phosphate from 2- or 3-phosphoglycerate, generating an enzyme-bound phosphoserine intermediate, followed by a phosphotransferase reaction as the phosphate is transferred from the enzyme back to the glycerate moiety. The structure also allowed the assignment of the function of the two domains of the enzyme, one of which participates in the phosphatase reaction and formation of the phosphoserine enzyme intermediate, with the other involved in the phosphotransferase reaction regenerating phosphoglycerate. Significant structural similarity has also been found between the active site of the iPGM domain catalyzing the phosphatase reaction and Escherichia coli alkaline phosphatase.
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==About this Structure==
==About this Structure==
1EQJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. The following page contains interesting information on the relation of 1EQJ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb50_1.html The Glycolytic Enzymes]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQJ OCA].
1EQJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. The following page contains interesting information on the relation of 1EQJ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb50_1.html The Glycolytic Enzymes]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQJ OCA].
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==Reference==
 
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Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus. Crystal structure of the complex with 2-phosphoglycerate., Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G, J Biol Chem. 2000 Jul 28;275(30):23146-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10764795 10764795]
 
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Phosphoglycerate mutase]]
[[Category: Phosphoglycerate mutase]]
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[[Category: Setlow, P.]]
[[Category: Setlow, P.]]
[[Category: Alpha/beta-type structure]]
[[Category: Alpha/beta-type structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:24:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:14:31 2008''

Revision as of 14:14, 26 June 2008

Image:1eqj.png

Template:STRUCTURE 1eqj

CRYSTAL STRUCTURE OF PHOSPHOGLYCERATE MUTASE FROM BACILLUS STEAROTHERMOPHILUS COMPLEXED WITH 2-PHOSPHOGLYCERATE


Template:ABSTRACT 10764795

About this Structure

1EQJ is a Single protein structure of sequence from Geobacillus stearothermophilus. The following page contains interesting information on the relation of 1EQJ with [The Glycolytic Enzymes]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:14:31 2008

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