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1ffy

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[[Image:1ffy.gif|left|200px]]
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[[Image:1ffy.png|left|200px]]
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==Overview==
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{{ABSTRACT_10446055}}
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Isoleucyl-transfer RNA (tRNA) synthetase (IleRS) joins Ile to tRNA(Ile) at its synthetic active site and hydrolyzes incorrectly acylated amino acids at its editing active site. The 2.2 angstrom resolution crystal structure of Staphylococcus aureus IleRS complexed with tRNA(Ile) and Mupirocin shows the acceptor strand of the tRNA(Ile) in the continuously stacked, A-form conformation with the 3' terminal nucleotide in the editing active site. To position the 3' terminus in the synthetic active site, the acceptor strand must adopt the hairpinned conformation seen in tRNA(Gln) complexed with its synthetase. The amino acid editing activity of the IleRS may result from the incorrect products shuttling between the synthetic and editing active sites, which is reminiscent of the editing mechanism of DNA polymerases.
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==About this Structure==
==About this Structure==
1FFY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. The following page contains interesting information on the relation of 1FFY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb16_1.html Aminoacyl-tRNA Synthetases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFY OCA].
1FFY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. The following page contains interesting information on the relation of 1FFY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb16_1.html Aminoacyl-tRNA Synthetases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFY OCA].
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==Reference==
 
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Insights into editing from an ile-tRNA synthetase structure with tRNAile and mupirocin., Silvian LF, Wang J, Steitz TA, Science. 1999 Aug 13;285(5430):1074-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10446055 10446055]
 
[[Category: Aminoacyl-tRNA Synthetases]]
[[Category: Aminoacyl-tRNA Synthetases]]
[[Category: Isoleucine--tRNA ligase]]
[[Category: Isoleucine--tRNA ligase]]
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[[Category: Mupirocin]]
[[Category: Mupirocin]]
[[Category: Staphylococcus aureus,protein-rna complex]]
[[Category: Staphylococcus aureus,protein-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:16:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:18:44 2008''

Revision as of 14:18, 26 June 2008

Image:1ffy.png

Template:STRUCTURE 1ffy

INSIGHTS INTO EDITING FROM AN ILE-TRNA SYNTHETASE STRUCTURE WITH TRNA(ILE) AND MUPIROCIN


Template:ABSTRACT 10446055

About this Structure

1FFY is a Single protein structure of sequence from Staphylococcus aureus. The following page contains interesting information on the relation of 1FFY with [Aminoacyl-tRNA Synthetases]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:18:44 2008

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