1ik9

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[[Image:1ik9.jpg|left|200px]]
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[[Image:1ik9.png|left|200px]]
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==Overview==
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{{ABSTRACT_11702069}}
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A complex of two proteins, Xrcc4 and DNA ligase IV, plays a fundamental role in DNA non-homologous end joining (NHEJ), a cellular function required for double-strand break repair and V(D)J recombination. Here we report the crystal structure of human Xrcc4 bound to a polypeptide that corresponds to the DNA ligase IV sequence linking its two BRCA1 C-terminal (BRCT) domains. In the complex, a single ligase chain binds asymmetrically to an Xrcc4 dimer. The helical tails of Xrcc4 undergo a substantial conformational change relative to the uncomplexed protein, forming a coiled coil that unwinds upon ligase binding, leading to a flat interaction surface. A buried network of charged hydrogen bonds surrounded by extensive hydrophobic contacts explains the observed tightness of the interaction. The strong conservation of residues at the interface between the two proteins provides evidence that the observed mode of interaction has been maintained in NHEJ throughout evolution.
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==Disease==
==Disease==
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==About this Structure==
==About this Structure==
1IK9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1IK9 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IK9 OCA].
1IK9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1IK9 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IK9 OCA].
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==Reference==
 
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Crystal structure of an Xrcc4-DNA ligase IV complex., Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L, Nat Struct Biol. 2001 Dec;8(12):1015-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11702069 11702069]
 
[[Category: DNA Ligase]]
[[Category: DNA Ligase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Double-strand break repair]]
[[Category: Double-strand break repair]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:05:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:33:16 2008''

Revision as of 14:33, 26 June 2008

Image:1ik9.png

Template:STRUCTURE 1ik9

CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX


Template:ABSTRACT 11702069

Disease

Known disease associated with this structure: LIG4 syndrome OMIM:[601837], Multiple myeloma, resistance to OMIM:[601837]

About this Structure

1IK9 is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1IK9 with [DNA Ligase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:33:16 2008

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OCA

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