1k8t

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[[Image:1k8t.gif|left|200px]]
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[[Image:1k8t.png|left|200px]]
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==Overview==
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{{ABSTRACT_11807546}}
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Oedema factor, a calmodulin-activated adenylyl cyclase, is important in the pathogenesis of anthrax. Here we report the X-ray structures of oedema factor with and without bound calmodulin. Oedema factor shares no significant structural homology with mammalian adenylyl cyclases or other proteins. In the active site, 3'-deoxy-ATP and a single metal ion are well positioned for catalysis with histidine 351 as the catalytic base. This mechanism differs from the mechanism of two-metal-ion catalysis proposed for mammalian adenylyl cyclases. Four discrete regions of oedema factor form a surface that recognizes an extended conformation of calmodulin, which is very different from the collapsed conformation observed in other structures of calmodulin bound to effector peptides. On calmodulin binding, an oedema factor helical domain of relative molecular mass 15,000 undergoes a 15 A translation and a 30 degrees rotation away from the oedema factor catalytic core, which stabilizes a disordered loop and leads to enzyme activation. These allosteric changes provide the first molecular details of how calmodulin modulates one of its targets.
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==About this Structure==
==About this Structure==
1K8T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The following page contains interesting information on the relation of 1K8T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8T OCA].
1K8T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The following page contains interesting information on the relation of 1K8T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8T OCA].
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==Reference==
 
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Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin., Drum CL, Yan SZ, Bard J, Shen YQ, Lu D, Soelaiman S, Grabarek Z, Bohm A, Tang WJ, Nature. 2002 Jan 24;415(6870):396-402. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11807546 11807546]
 
[[Category: Adenylate cyclase]]
[[Category: Adenylate cyclase]]
[[Category: Anthrax Toxin]]
[[Category: Anthrax Toxin]]
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[[Category: Calmodulin]]
[[Category: Calmodulin]]
[[Category: Edema factor]]
[[Category: Edema factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:26:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:37:51 2008''

Revision as of 14:37, 26 June 2008

Image:1k8t.png

Template:STRUCTURE 1k8t

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)


Template:ABSTRACT 11807546

About this Structure

1K8T is a Single protein structure of sequence from Bacillus anthracis. The following page contains interesting information on the relation of 1K8T with [Anthrax Toxin]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:37:51 2008

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