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2a7u

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[[Image:2a7u.gif|left|200px]]
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[[Image:2a7u.png|left|200px]]
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==Overview==
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{{ABSTRACT_16128580}}
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A critical point of interaction between F(1) and F(0) in the bacterial F(1)F(0)-ATP synthase is formed by the alpha and delta subunits. Previous work has shown that the N-terminal domain (residues 3-105) of the delta subunit forms a 6 alpha-helix bundle [Wilkens, S., Dunn, S. D., Chandler, J., Dahlquist, F. W., and Capaldi, R. A. (1997) Nat. Struct. Biol. 4, 198-201] and that the majority of the binding energy between delta and F(1) is provided by the interaction between the N-terminal 22 residues of the alpha- and N-terminal domain of the delta subunit [Weber, J., Muharemagic, A., Wilke-Mounts, S., and Senior, A. E. (2003) J. Biol. Chem. 278, 13623-13626]. We have now analyzed a 1:1 complex of the delta-subunit N-terminal domain and a peptide comprising the N-terminal 22 residues of the alpha subunit by heteronuclear protein NMR spectroscopy. A comparison of the chemical-shift values of delta-subunit residues with and without alpha N-terminal peptide bound indicates that the binding interface on the N-terminal domain of the delta subunit is formed by alpha helices I and V. NOE cross-peak patterns in 2D (12)C/(12)C-filtered NOESY spectra of the (13)C-labeled delta-subunit N-terminal domain in complex with unlabeled peptide verify that residues 8-18 in the alpha-subunit N-terminal peptide are folded as an alpha helix when bound to delta N-terminal domain. On the basis of intermolecular contacts observed in (12)C/(13)C-filtered NOESY experiments, we describe structural details of the interaction of the delta-subunit N-terminal domain with the alpha-subunit N-terminal alpha helix.
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==About this Structure==
==About this Structure==
2A7U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 2A7U with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb72_1.html ATP Synthase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7U OCA].
2A7U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 2A7U with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb72_1.html ATP Synthase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7U OCA].
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==Reference==
 
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Structural characterization of the interaction of the delta and alpha subunits of the Escherichia coli F1F0-ATP synthase by NMR spectroscopy., Wilkens S, Borchardt D, Weber J, Senior AE, Biochemistry. 2005 Sep 6;44(35):11786-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16128580 16128580]
 
[[Category: ATP Synthase]]
[[Category: ATP Synthase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Wilkens, S.]]
[[Category: Wilkens, S.]]
[[Category: Alpha helix bundle]]
[[Category: Alpha helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:43:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:11:26 2008''

Revision as of 15:11, 26 June 2008

Image:2a7u.png

Template:STRUCTURE 2a7u

NMR solution structure of the E.coli F-ATPase delta subunit N-terminal domain in complex with alpha subunit N-terminal 22 residues


Template:ABSTRACT 16128580

About this Structure

2A7U is a Protein complex structure of sequences from Escherichia coli. The following page contains interesting information on the relation of 2A7U with [ATP Synthase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:11:26 2008

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