2d1t

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[[Image:2d1t.gif|left|200px]]
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==Overview==
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{{ABSTRACT_16541080}}
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Fireflies communicate with each other by emitting yellow-green to yellow-orange brilliant light. The bioluminescence reaction, which uses luciferin, Mg-ATP and molecular oxygen to yield an electronically excited oxyluciferin species, is carried out by the enzyme luciferase. Visible light is emitted during relaxation of excited oxyluciferin to its ground state. The high quantum yield of the luciferin/luciferase reaction and the change in bioluminescence colour caused by subtle structural differences in luciferase have attracted much research interest. In fact, a single amino acid substitution in luciferase changes the emission colour from yellow-green to red. Although the crystal structure of luciferase from the North American firefly (Photinus pyralis) has been described, the detailed mechanism for the bioluminescence colour change is still unclear. Here we report the crystal structures of wild-type and red mutant (S286N) luciferases from the Japanese Genji-botaru (Luciola cruciata) in complex with a high-energy intermediate analogue, 5'-O-[N-(dehydroluciferyl)-sulfamoyl]adenosine (DLSA). Comparing these structures to those of the wild-type luciferase complexed with AMP plus oxyluciferin (products) reveals a significant conformational change in the wild-type enzyme but not in the red mutant. This conformational change involves movement of the hydrophobic side chain of Ile 288 towards the benzothiazole ring of DLSA. Our results indicate that the degree of molecular rigidity of the excited state of oxyluciferin, which is controlled by a transient movement of Ile 288, determines the colour of bioluminescence during the emission reaction.
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==About this Structure==
==About this Structure==
2D1T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Luciola_cruciata Luciola cruciata]. The following page contains interesting information on the relation of 2D1T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1T OCA].
2D1T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Luciola_cruciata Luciola cruciata]. The following page contains interesting information on the relation of 2D1T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1T OCA].
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==Reference==
 
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Structural basis for the spectral difference in luciferase bioluminescence., Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H, Nature. 2006 Mar 16;440(7082):372-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16541080 16541080]
 
[[Category: Luciferase]]
[[Category: Luciferase]]
[[Category: Luciola cruciata]]
[[Category: Luciola cruciata]]
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:32:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:15:03 2008''

Revision as of 15:15, 26 June 2008

Template:STRUCTURE 2d1t

Crystal structure of the thermostable Japanese Firefly Luciferase red-color emission S286N mutant complexed with High-energy intermediate analogue


Template:ABSTRACT 16541080

About this Structure

2D1T is a Single protein structure of sequence from Luciola cruciata. The following page contains interesting information on the relation of 2D1T with [Luciferase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:15:03 2008

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