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2f1m

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[[Image:2f1m.gif|left|200px]]
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[[Image:2f1m.png|left|200px]]
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==Overview==
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{{ABSTRACT_16531241}}
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Intrinsic resistance to multiple drugs in many gram-negative bacterial pathogens is conferred by resistance nodulation cell division efflux pumps, which are composed of three essential components as typified by the extensively characterized Escherichia coli AcrA-AcrB-TolC system. The inner membrane drug:proton antiporter AcrB and the outer membrane channel TolC export chemically diverse compounds out of the bacterial cell, and require the activity of the third component, the periplasmic protein AcrA. The crystal structures of AcrB and TolC have previously been determined, and we complete the molecular picture of the efflux system by presenting the structure of a stable fragment of AcrA. The AcrA fragment resembles the elongated sickle shape of its homolog Pseudomonas aeruginosa MexA, being composed of three domains: beta-barrel, lipoyl, and alpha-helical hairpin. Notably, unsuspected conformational flexibility in the alpha-helical hairpin domain of AcrA is observed, which has potential mechanistic significance in coupling between AcrA conformations and TolC channel opening.
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==About this Structure==
==About this Structure==
2F1M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F1M OCA].
2F1M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F1M OCA].
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==Reference==
 
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Conformational flexibility in the multidrug efflux system protein AcrA., Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P, Structure. 2006 Mar;14(3):577-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16531241 16531241]
 
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Helical hairpin]]
[[Category: Helical hairpin]]
[[Category: Lipoyl domain]]
[[Category: Lipoyl domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:21:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:15:51 2008''

Revision as of 15:15, 26 June 2008

Image:2f1m.png

Template:STRUCTURE 2f1m

Conformational flexibility in the multidrug efflux system protein AcrA


Template:ABSTRACT 16531241

About this Structure

2F1M is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:15:51 2008

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