7dfr

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==Overview==
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{{ABSTRACT_2185835}}
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The crystal structure of dihydrofolate reductase (EC 1.5.1.3) from Escherichia coli has been solved as the binary complex with NADP+ (the holoenzyme) and as the ternary complex with NADP+ and folate. The Bragg law resolutions of the structures are 2.4 and 2.5 A, respectively. The new crystal forms are nonisomorphous with each other and with the methotrexate binary complex reported earlier [Bolin, J. T., Filman, D. J., Matthews, D. A., Hamlin, R. C., &amp; Kraut, J. (1982) J. Biol. Chem. 257, 13650-13662]. In general, NADP+ and folate binding conform to predictions, but the nicotinamide moiety of NADP+ is disordered in the holoenzyme and ordered in the ternary complex. A mobile loop (residues 16-20) involved in binding the nicotinamide is also disordered in the holoenzyme. We report a detailed analysis of the binding interactions for both ligands, paying special attention to several apparently strained interactions that may favor the transition state for hydride transfer. Hypothetical models are presented for the binding of 7,8-dihydrofolate in the Michaelis complex and for the transition-state complex.
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==About this Structure==
==About this Structure==
7DFR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 7DFR with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb34_1.html Dihydrofolate Reductase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DFR OCA].
7DFR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 7DFR with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb34_1.html Dihydrofolate Reductase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DFR OCA].
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==Reference==
 
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Crystal structures of Escherichia coli dihydrofolate reductase: the NADP+ holoenzyme and the folate.NADP+ ternary complex. Substrate binding and a model for the transition state., Bystroff C, Oatley SJ, Kraut J, Biochemistry. 1990 Apr 3;29(13):3263-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2185835 2185835]
 
[[Category: Dihydrofolate Reductase]]
[[Category: Dihydrofolate Reductase]]
[[Category: Dihydrofolate reductase]]
[[Category: Dihydrofolate reductase]]
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[[Category: Oatley, S J.]]
[[Category: Oatley, S J.]]
[[Category: Oxido-reductase]]
[[Category: Oxido-reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:31:13 2008''

Revision as of 15:31, 26 June 2008

Image:7dfr.png

Template:STRUCTURE 7dfr

CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. THE NADP+ HOLOENZYME AND THE FOLATE(DOT)NADP+ TERNARY COMPLEX. SUBSTRATE BINDING AND A MODEL FOR THE TRANSITION STATE


Template:ABSTRACT 2185835

About this Structure

7DFR is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 7DFR with [Dihydrofolate Reductase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:31:13 2008

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