8ruc

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[[Image:8ruc.gif|left|200px]]
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[[Image:8ruc.png|left|200px]]
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==Overview==
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{{ABSTRACT_8648644}}
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Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from spinach is a hexadecamer (L8S8, Mr = 550,000) consisting of eight large (L, 475 residues) and eight small subunits (S, 123 residues). High-resolution data collection on crystals with large unit cells is not a trivial task due to the effect of radiation damage and the large number of overlapping reflections when conventional data collection methods are used. In order to minimise these effects, data on rubisco were collected with a giant Weissenberg camera at long crystal to image-plate distances at the synchrotron of the Photon Factory, Japan. Relative to conventional data sets, this experimental arrangement allowed a 20 to 30-fold reduction of the X-ray dose/exposure time for data collection. This paper describes the refined 1.6 A crystal structure of activated rubisco complexed with a transition state analogue, 2-carboxyarabinitol-bisphosphate. The crystallographic asymmetric unit contains an L4S4 unit, representing half of the molecule. The structure presented here is currently the highest resolution structure for any protein of comparable size. Refinement of the model was carried out by restrained least squares techniques without non-crystallographic symmetry averaging. The results show that all L and S subunits have identical three-dimensional structures, and their arrangement within the hexadecamer has no intrinsic asymmetry. A detailed analysis of the high-resolution maps identified 30 differences in the sequence of the small subunit, indicating a larger than usual heterogeneity for this nuclear encoded protein in spinach. No such differences were found in the sequence of the chloroplast encoded large subunit. The transition state analogue is in the cis conformation at the active site suggesting a key role for the carbamate of Lys201 in catalysis. Analysis of the active site around the catalytically essential magnesium ion further indicates that residues in the second liganding sphere of the metal play a role in fine-tuning the acid-base character and the position of the residues directly liganded to the metal.
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==About this Structure==
==About this Structure==
8RUC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=8rub 8rub]. The following page contains interesting information on the relation of 8RUC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RUC OCA].
8RUC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=8rub 8rub]. The following page contains interesting information on the relation of 8RUC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RUC OCA].
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==Reference==
 
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Large structures at high resolution: the 1.6 A crystal structure of spinach ribulose-1,5-bisphosphate carboxylase/oxygenase complexed with 2-carboxyarabinitol bisphosphate., Andersson I, J Mol Biol. 1996 May 31;259(1):160-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8648644 8648644]
 
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Ribulose-bisphosphate carboxylase]]
[[Category: Ribulose-bisphosphate carboxylase]]
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[[Category: Photorespiration]]
[[Category: Photorespiration]]
[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:51:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:31:54 2008''

Revision as of 15:31, 26 June 2008

Image:8ruc.png

Template:STRUCTURE 8ruc

ACTIVATED SPINACH RUBISCO COMPLEXED WITH 2-CARBOXYARABINITOL BISPHOSPHATE


Template:ABSTRACT 8648644

About this Structure

8RUC is a Protein complex structure of sequences from Spinacia oleracea. This structure supersedes the now removed PDB entry 8rub. The following page contains interesting information on the relation of 8RUC with [Rubisco]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:31:54 2008

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