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113d

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{{STRUCTURE_113d| PDB=113d | SCENE= }}
{{STRUCTURE_113d| PDB=113d | SCENE= }}
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'''THE STRUCTURE OF GUANOSINE-THYMIDINE MISMATCHES IN B-DNA AT 2.5 ANGSTROMS RESOLUTION'''
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===THE STRUCTURE OF GUANOSINE-THYMIDINE MISMATCHES IN B-DNA AT 2.5 ANGSTROMS RESOLUTION===
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==Overview==
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The structure of the deoxyoligomer d(C-G-C-G-A-A-T-T-T-G-C-G) was determined at 2.5-A resolution by single crystal x-ray diffraction techniques. The final R factor is 18% with the location of 71 water molecules. The oligomer crystallizes in a B-DNA-type conformation, with two strands interacting to form a dodecamer duplex. The double helix consists of four A X T and six G X C Watson-Crick base pairs and two G X T mismatches. The G X T pairs adopt a "wobble" structure with the thymine projecting into the major groove and the guanine into the minor groove. The mispairs are accommodated in the normal double helix by small adjustments in the conformation of the sugar phosphate backbone. A comparison with the isomorphous parent compound containing only Watson-Crick base pairs shows that any changes in the structure induced by the presence of G X T mispairs are highly localized. The global conformation of the duplex is conserved. The G X T mismatch has already been studied by x-ray techniques in A and Z helices where similar results were found. The geometry of the mispair is essentially identical in all structures so far examined, irrespective of the DNA conformation. The hydration is also similar with solvent molecules bridging the functional groups of the bases via hydrogen bonds. Hydration may be an important factor in stabilizing G X T mismatches. A characteristic of Watson-Crick paired A X T and G X C bases is the pseudo 2-fold symmetry axis in the plane of the base pairs. The G X T wobble base pair is pronouncedly asymmetric. This asymmetry, coupled with the disposition of functional groups in the major and minor grooves, provides a number of features which may contribute to the recognition of the mismatch by repair enzymes.
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(as it appears on PubMed at http://www.pubmed.gov), where 3611072 is the PubMed ID number.
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{{ABSTRACT_PUBMED_3611072}}
==About this Structure==
==About this Structure==
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[[Category: Double helix]]
[[Category: Double helix]]
[[Category: Mismatched]]
[[Category: Mismatched]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:26:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:22:57 2008''

Revision as of 12:23, 30 June 2008

Image:113d.png

Template:STRUCTURE 113d

THE STRUCTURE OF GUANOSINE-THYMIDINE MISMATCHES IN B-DNA AT 2.5 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 3611072

About this Structure

Full crystallographic information is available from OCA.

Reference

The structure of guanosine-thymidine mismatches in B-DNA at 2.5-A resolution., Hunter WN, Brown T, Kneale G, Anand NN, Rabinovich D, Kennard O, J Biol Chem. 1987 Jul 25;262(21):9962-70. PMID:3611072

Page seeded by OCA on Mon Jun 30 15:22:57 2008

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