From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:139d.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:139d.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_139d| PDB=139d | SCENE= }} | | {{STRUCTURE_139d| PDB=139d | SCENE= }} |
| | | | |
| - | '''SOLUTION STRUCTURE OF A PARALLEL-STRANDED G-QUADRUPLEX DNA'''
| + | ===SOLUTION STRUCTURE OF A PARALLEL-STRANDED G-QUADRUPLEX DNA=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | This paper reports on the solution structure of a parallel-stranded G-quadruplex formed by the Tetrahymena telomeric sequence d(T-T-G-G-G-G-T) whose NMR parameters in potassium cation containing solution were previously published from our laboratory. The structure was determined by combining a quantitative analysis of the NMR data with molecular dynamics calculations including relaxation matrix refinement. The combined NMR-computational approach yielded a set of seven distance-refined structures with pairwise RMSDs ranging from 0.66 to 1.30 A for the central G-G-G-G tetranucleotide segment. Four of the seven structures were refined further using complete relaxation-matrix calculations to yield solution structures with pairwise RMSDs ranging from 0.64 to 1.04 A for the same tetranucleotide segment. The R-factors also decreased on proceeding from the distance-refined to relaxation matrix-refined structures. The four strands of the G-quadruplex are aligned in parallel and are related by a 4-fold symmetry axis coincident with the helix axis. Individual guanines from each strand form planar G.G.G.G tetrad arrangements with each tetrad stabilized by eight hydrogen bonds involving the Watson-Crick and Hoogsteen edges of the guanine bases. All guanines adopt anti glycosidic torsion angles and S type sugar puckers in this right-handed parallel-stranded G-quadruplex structure. The four G.G.G.G tetrad planes stack on each other with minimal overlap of adjacent guanine base planes within individual strands. The thymine residues are under-defined in the solution structure of the d(T-T-G-G-G-G-T) G-quadruplex and sample amongst multiple conformations in solution.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8263919}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8263919 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_8263919}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=139D OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=139D OCA]. |
| | | | |
| | ==Reference== | | ==Reference== |
| Line 26: |
Line 30: |
| | [[Category: G-quadruplex]] | | [[Category: G-quadruplex]] |
| | [[Category: Nmr]] | | [[Category: Nmr]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:29:28 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:28:45 2008'' |
Revision as of 12:28, 30 June 2008
Template:STRUCTURE 139d
SOLUTION STRUCTURE OF A PARALLEL-STRANDED G-QUADRUPLEX DNA
Template:ABSTRACT PUBMED 8263919
About this Structure
Full experimental information is available from OCA.
Reference
Solution structure of a parallel-stranded G-quadruplex DNA., Wang Y, Patel DJ, J Mol Biol. 1993 Dec 20;234(4):1171-83. PMID:8263919
Page seeded by OCA on Mon Jun 30 15:28:45 2008
