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| | {{STRUCTURE_143d| PDB=143d | SCENE= }} | | {{STRUCTURE_143d| PDB=143d | SCENE= }} |
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| - | '''SOLUTION STRUCTURE OF THE HUMAN TELOMERIC REPEAT D(AG3[T2AG3]3) OF THE G-QUADRUPLEX'''
| + | ===SOLUTION STRUCTURE OF THE HUMAN TELOMERIC REPEAT D(AG3[T2AG3]3) OF THE G-QUADRUPLEX=== |
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| - | ==Overview==
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| - | BACKGROUND: Repeats of Gn sequences are detected as single strand overhangs at the ends of eukaryotic chromosomes together with associated binding proteins. Such telomere sequences have been implicated in the replication and maintenance of chromosomal termini. They may also mediate chromosomal organization and association during meiosis and mitosis. RESULTS: We have determined the three-dimensional solution structure of the human telomere sequence, d[AG3(T2AG3)3] in Na(+)-containing solution using a combined NMR, distance geometry and molecular dynamics approach (including relaxation matrix refinement). The sequence, which contains four AG3 repeats, folds intramolecularly into a G-tetraplex stabilized by three stacked G-tetrads which are connected by two lateral loops and a central diagonal loop. Of the four grooves that are formed, one is wide, two are of medium width and one is narrow. The alignment of adjacent G-G-G segments in parallel generates the two grooves of medium width whilst the antiparallel arrangement results in one wide and one narrow groove. Three of the four adenines stack on top of adjacent G-tetrads while the majority of the thymines sample multiple conformations. CONCLUSIONS: The availability of the d[AG3(T2AG3)3] solution structure containing four AG3 human telomeric repeats should permit the rational design of ligands that recognize and bind with specificity and affinity to the individual grooves of the G-tetraplex, as well as to either end containing the diagonal and lateral loops. Such ligands could modulate the equilibrium between folded G-tetraplex structures and their unfolded extended counterparts.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8081740}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8081740 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8081740}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=143D OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=143D OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Human telomeric sequence]] | | [[Category: Human telomeric sequence]] |
| | [[Category: Nmr]] | | [[Category: Nmr]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:29:57 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:29:41 2008'' |
Revision as of 12:29, 30 June 2008
Template:STRUCTURE 143d
SOLUTION STRUCTURE OF THE HUMAN TELOMERIC REPEAT D(AG3[T2AG3]3) OF THE G-QUADRUPLEX
Template:ABSTRACT PUBMED 8081740
About this Structure
Full experimental information is available from OCA.
Reference
Solution structure of the human telomeric repeat d[AG3(T2AG3)3] G-tetraplex., Wang Y, Patel DJ, Structure. 1993 Dec 15;1(4):263-82. PMID:8081740
Page seeded by OCA on Mon Jun 30 15:29:41 2008
