From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:146d.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:146d.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_146d| PDB=146d | SCENE= }} | | {{STRUCTURE_146d| PDB=146d | SCENE= }} |
| | | | |
| - | '''SOLUTION STRUCTURE OF THE MITHRAMYCIN DIMER-DNA COMPLEX'''
| + | ===SOLUTION STRUCTURE OF THE MITHRAMYCIN DIMER-DNA COMPLEX=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | We have characterized the NMR parameters for the complexes formed by the Mg(2+)-coordinated mithramycin dimer with self-complementary d(T-G-G-C-C-A) and d(T-C-G-C-G-A) duplexes. The solution structure of the latter complex has been determined using a combined NMR-molecular dynamics study including relaxation matrix refinement. The Mg(2+)-coordinated mithramycin dimer-d(T-C-G-C-G-A) complex exhibits a 2-fold center of symmetry with the divalent cation coordinated aglycons positioned opposite the central (G3-C4).(G3-C4) segment such that the aglycon C8 hydroxyl oxygens form symmetrical sequence-specific hydrogen bonds to guanine amino protons in the complex. The C-D-E trisaccharide segments of each monomer in the mithramycin dimer adopt extended conformations, are positioned inside the minor groove, and are directed toward either end of the duplex. The C-D saccharide component of one monomer and the aglycon of the other monomer in the mithramycin dimer share a widened minor groove with the hydrophobic edges of the C and D sugars interacting with individual strands of the duplex. The E-sugar ring is positioned in the floor of the minor groove, and its hydroxyl-bearing face interacts with both strands of the duplex through hydrogen-bonding and hydrophobic intermolecular interactions. The A-B disaccharide and the hydrophilic side chain form intermolecular contacts with the sugar-phosphate backbone in the complex. The antiparallel alignment of divalent cation coordinated monomers in the mithramycin dimer results in the two outwardly directed C-D-E trisaccharide segments generating a right-handed continuous hexasaccharide domain that spans six base pairs in the minor groove of the duplex. The solution structure of the mithramycin dimer-DNA complex reported in this study and the solution structure of the chromomycin dimer-DNA complex reported previously [Gao, X., Mirau, P., & Patel, D. J. (1992) J. Mol. Biol. 223, 259-279] show global similarities, as well as local differences that are of interest. All four nucleotides in the tetranucleotide segment of the duplex centered about the sequence-specific (G-C).(G-C) step adopt A-DNA sugar puckers and glycosidic torsion angles in the chromomycin dimer-DNA complex, while only the central cytidine adopts an A-DNA sugar pucker and glycosidic torsion angle in the mithramycin dimer-DNA complex.(ABSTRACT TRUNCATED AT 400 WORDS)
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8329387}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8329387 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_8329387}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=146D OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=146D OCA]. |
| | | | |
| | ==Reference== | | ==Reference== |
| Line 25: |
Line 29: |
| | [[Category: Mithramycin dimer]] | | [[Category: Mithramycin dimer]] |
| | [[Category: Nmr]] | | [[Category: Nmr]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:30:13 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:30:15 2008'' |
Revision as of 12:30, 30 June 2008
Template:STRUCTURE 146d
SOLUTION STRUCTURE OF THE MITHRAMYCIN DIMER-DNA COMPLEX
Template:ABSTRACT PUBMED 8329387
About this Structure
Full experimental information is available from OCA.
Reference
Solution structure of the mithramycin dimer-DNA complex., Sastry M, Patel DJ, Biochemistry. 1993 Jul 6;32(26):6588-604. PMID:8329387
Page seeded by OCA on Mon Jun 30 15:30:15 2008
