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189l

From Proteopedia

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[[Image:189l.gif|left|200px]]
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[[Image:189l.png|left|200px]]
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{{STRUCTURE_189l| PDB=189l | SCENE= }}
{{STRUCTURE_189l| PDB=189l | SCENE= }}
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'''ENHANCEMENT OF PROTEIN STABILITY BY THE COMBINATION OF POINT MUTATIONS IN T4 LYSOZYME IS ADDITIVE'''
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===ENHANCEMENT OF PROTEIN STABILITY BY THE COMBINATION OF POINT MUTATIONS IN T4 LYSOZYME IS ADDITIVE===
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==Overview==
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A number of mutations have been shown previously to stabilize T4 lysozyme. By combining up to seven such mutations in the same protein, the melting temperature was incrementally increased by up to 8.3 degrees C at pH 5.4 (delta delta G = 3.6 kcal/mol). This shows that it is possible to engineer a protein of enhanced thermostability by combining a series of rationally designed point mutations. It is also shown that this stabilization is achieved with only minor, localized changes in the structure of the protein. This is consistent with the observation that the change in stability of each of the multiple mutants is, in each case, additive, i.e. equal to the sum of the stability changes associated with the constituent single mutants. One of the seven substitutions, Asn116--&gt;Asp, changes a residue that participates in substrate binding; not surprisingly, it causes a significant loss in activity. Ignoring this mutation, there is a gradual reduction in activity as successively more mutations are combined.
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The line below this paragraph, {{ABSTRACT_PUBMED_8771182}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8771182 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8771182}}
==About this Structure==
==About this Structure==
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[[Category: Matthews, B W.]]
[[Category: Matthews, B W.]]
[[Category: Zhang, X J.]]
[[Category: Zhang, X J.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:34:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:38:58 2008''

Revision as of 12:39, 30 June 2008

Image:189l.png

Template:STRUCTURE 189l

ENHANCEMENT OF PROTEIN STABILITY BY THE COMBINATION OF POINT MUTATIONS IN T4 LYSOZYME IS ADDITIVE

Template:ABSTRACT PUBMED 8771182

About this Structure

189L is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Enhancement of protein stability by the combination of point mutations in T4 lysozyme is additive., Zhang XJ, Baase WA, Shoichet BK, Wilson KP, Matthews BW, Protein Eng. 1995 Oct;8(10):1017-22. PMID:8771182

Page seeded by OCA on Mon Jun 30 15:38:58 2008

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