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1a1f

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[[Image:1a1f.gif|left|200px]]
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{{STRUCTURE_1a1f| PDB=1a1f | SCENE= }}
{{STRUCTURE_1a1f| PDB=1a1f | SCENE= }}
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'''DSNR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GACC SITE)'''
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===DSNR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GACC SITE)===
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==Overview==
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BACKGROUND: Zinc fingers of the Cys2-His2 class comprise one of the largest families of eukaryotic DNA-binding motifs and recognize a diverse set of DNA sequences. These proteins have a relatively simple modular structure and key base contacts are typically made by a few residues from each finger. These features make the zinc finger motif an attractive system for designing novel DNA-binding proteins and for exploring fundamental principles of protein-DNA recognition. RESULTS: Here we report the X-ray crystal structures of zinc finger-DNA complexes involving three variants of Zif268, with multiple changes in the recognition helix of finger one. We describe the structure of each of these three-finger peptides bound to its corresponding target site. To help elucidate the differential basis for site-specific recognition, the structures of four other complexes containing various combinations of these peptides with alternative binding sites have also been determined. CONCLUSIONS: The protein-DNA contacts observed in these complexes reveal the basis for the specificity demonstrated by these Zif268 variants. Many, but not all, of the contacts can be rationalized in terms of a recognition code, but the predictive value of such a code is limited. The structures illustrate how modest changes in the docking arrangement accommodate the new sidechain-base and sidechain-phosphate interactions. Such adaptations help explain the versatility of naturally occurring zinc finger proteins and their utility in design.
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The line below this paragraph, {{ABSTRACT_PUBMED_9562555}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9562555 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9562555}}
==About this Structure==
==About this Structure==
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[[Category: Dna-binding protein]]
[[Category: Dna-binding protein]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:39:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:47:01 2008''

Revision as of 12:47, 30 June 2008

Image:1a1f.png

Template:STRUCTURE 1a1f

DSNR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GACC SITE)

Template:ABSTRACT PUBMED 9562555

About this Structure

1A1F is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

High-resolution structures of variant Zif268-DNA complexes: implications for understanding zinc finger-DNA recognition., Elrod-Erickson M, Benson TE, Pabo CO, Structure. 1998 Apr 15;6(4):451-64. PMID:9562555

Page seeded by OCA on Mon Jun 30 15:47:01 2008

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