1a2s

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{{STRUCTURE_1a2s| PDB=1a2s | SCENE= }}
{{STRUCTURE_1a2s| PDB=1a2s | SCENE= }}
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'''THE SOLUTION NMR STRUCTURE OF OXIDIZED CYTOCHROME C6 FROM THE GREEN ALGA MONORAPHIDIUM BRAUNII, MINIMIZED AVERAGE STRUCTURE'''
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===THE SOLUTION NMR STRUCTURE OF OXIDIZED CYTOCHROME C6 FROM THE GREEN ALGA MONORAPHIDIUM BRAUNII, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constraints, 1109 of which were meaningful, together with 288 pseudocontact shifts, have been used to determine the structure in solution. This is represented as a family of 40 structures which have been energy minimized. The rmsd values with respect to the mean structure are 0.57 +/- 0.08 and 0.94 +/- 0.09 A for the backbone and heavy atoms, respectively. The structure has been found to be very similar to that of the reduced form, except for a rearrangement in propionate 7, a feature which has been observed in all c-type cytochromes investigated so far. Such a feature could be relevant for the efficiency of the electron transfer pathway with either the oxidizing or the reducing partners. Other differences in the oxidation states have been noted in the region proposed to be involved in the interaction with the physiological partners.
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{{ABSTRACT_PUBMED_9538000}}
==About this Structure==
==About this Structure==
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1A2S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Monoraphidium_braunii Monoraphidium braunii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2S OCA].
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1A2S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Monoraphidium_braunii Monoraphidium braunii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2S OCA].
==Reference==
==Reference==
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[[Category: Photosystem i]]
[[Category: Photosystem i]]
[[Category: Solution structure]]
[[Category: Solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:43:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:52:19 2008''

Revision as of 12:52, 30 June 2008

Image:1a2s.png

Template:STRUCTURE 1a2s

THE SOLUTION NMR STRUCTURE OF OXIDIZED CYTOCHROME C6 FROM THE GREEN ALGA MONORAPHIDIUM BRAUNII, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9538000

About this Structure

1A2S is a Single protein structure of sequence from Monoraphidium braunii. Full experimental information is available from OCA.

Reference

Solution structure of oxidized cytochrome c6 from the green alga Monoraphidium braunii., Banci L, Bertini I, De la Rosa MA, Koulougliotis D, Navarro JA, Walter O, Biochemistry. 1998 Apr 7;37(14):4831-43. PMID:9538000

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