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| - | [[Image:1a2v.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1a2v| PDB=1a2v | SCENE= }} | | {{STRUCTURE_1a2v| PDB=1a2v | SCENE= }} |
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| - | '''COPPER AMINE OXIDASE FROM HANSENULA POLYMORPHA'''
| + | ===COPPER AMINE OXIDASE FROM HANSENULA POLYMORPHA=== |
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| - | ==Overview==
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| - | The copper-containing amine oxidase from the yeast Hansenula polymorpha (YAO) has been crystallized and partially solved by molecular replacement. It catalyzes the oxidative deamination of primary amines by molecular oxygen to the corresponding aldehydes, ammonia and hydrogen peroxide. It contains a covalently bound redox cofactor, topa quinone, generated by post-translational modification of a single tyrosine side chain. The crystals of YAO are orthorhombic, with space-group symmetry P2(1)2(1)2(1) and unit-cell dimensions a = 138.8, b = 148.2, c = 234.0 A and diffract X-rays beyond 2.0 A resolution. Solution by molecular replacement using the E. coli amine oxidase structure [Parsons, Convery, Wilmot, Yadav, Blakeley, Corner, Philips, McPherson & Knowles (1995). Structure, 3, 1171-1184] as a search model reveals that there are three dimers in the asymmetric unit in a trigonal arrangement having 32 point-group symmetry. The solution agrees well with the self-rotation function of YAO. The non-crystallographic threefold axis lies parallel to a crystallographic twofold screw axis and each dimer has twofold symmetry. Phases from the refined model based on the molecular-replacement solution were used to solve one heavy-atom derivative. Model building from the unbiased isomorphous replacement phases is in progress. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15299901}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15299901 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15299901}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Topaquinone enzyme]] | | [[Category: Topaquinone enzyme]] |
| | [[Category: Tpq]] | | [[Category: Tpq]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:43:39 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:52:43 2008'' |
Revision as of 12:52, 30 June 2008
Template:STRUCTURE 1a2v
COPPER AMINE OXIDASE FROM HANSENULA POLYMORPHA
Template:ABSTRACT PUBMED 15299901
About this Structure
1A2V is a Single protein structure of sequence from Pichia angusta. Full crystallographic information is available from OCA.
Reference
Crystallographic study of yeast copper amine oxidase., Li R, Chen L, Cai D, Klinman JP, Mathews FS, Acta Crystallogr D Biol Crystallogr. 1997 Jul 1;53(Pt 4):364-70. PMID:15299901
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