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1a7f

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[[Image:1a7f.gif|left|200px]]
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{{Seed}}
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[[Image:1a7f.png|left|200px]]
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{{STRUCTURE_1a7f| PDB=1a7f | SCENE= }}
{{STRUCTURE_1a7f| PDB=1a7f | SCENE= }}
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'''INSULIN MUTANT B16 GLU, B24 GLY, DES-B30, NMR, 20 STRUCTURES'''
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===INSULIN MUTANT B16 GLU, B24 GLY, DES-B30, NMR, 20 STRUCTURES===
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==Overview==
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Despite years of effort to clarify the structural basis of insulin receptor binding no clear consensus has emerged. It is generally believed that insulin receptor binding is accompanied by some degree of conformational change in the carboxy-terminal of the insulin B-chain. In particular, while most substitutions for PheB24 lead to inactive species, glycine or D-amino acids are well tolerated in this position. Here we assess the conformation change by solving the solution structure of the biologically active (GluB16, GlyB24, desB30)-insulin mutant. The structure in aqueous solution at pH 8 reveals a subtle, albeit well-defined rearrangement of the C-terminal decapeptide involving a perturbation of the B20-23 turn, which allows the PheB25 residue to occupy the position normally taken up by PheB24 in native insulin. The new protein surface exposed rationalizes the receptor binding properties of a series of insulin analogs. We suggest that the structural switch is forced by the structure of the underlying core of species invariant residues and that an analogous rearrangement of the C-terminal of the B-chain occurs in native insulin on binding to its receptor.
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The line below this paragraph, {{ABSTRACT_PUBMED_9636695}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9636695 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9636695}}
==About this Structure==
==About this Structure==
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1A7F is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7F OCA].
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1A7F is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7F OCA].
==Reference==
==Reference==
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[[Category: Monomer]]
[[Category: Monomer]]
[[Category: Neutral ph]]
[[Category: Neutral ph]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:55:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:18:24 2008''

Revision as of 13:18, 30 June 2008

Image:1a7f.png

Template:STRUCTURE 1a7f

INSULIN MUTANT B16 GLU, B24 GLY, DES-B30, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 9636695

About this Structure

1A7F is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

A structural switch in a mutant insulin exposes key residues for receptor binding., Ludvigsen S, Olsen HB, Kaarsholm NC, J Mol Biol. 1998 May 29;279(1):1-7. PMID:9636695

Page seeded by OCA on Mon Jun 30 16:18:24 2008

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