1a84

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{{STRUCTURE_1a84| PDB=1a84 | SCENE= }}
{{STRUCTURE_1a84| PDB=1a84 | SCENE= }}
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'''NMR SOLUTION STRUCTURE OF A DNA DODECAMER DUPLEX CONTAINING A CIS-DIAMMINEPLATINUM(II) D(GPG) INTRASTRAND CROSS-LINK, THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN, 1 STRUCTURE'''
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===NMR SOLUTION STRUCTURE OF A DNA DODECAMER DUPLEX CONTAINING A CIS-DIAMMINEPLATINUM(II) D(GPG) INTRASTRAND CROSS-LINK, THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN, 1 STRUCTURE===
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==Overview==
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The structure of the DNA duplex dodecamer, d(CCTCTGGTCTCC. GGAGACCAGAGG), containing the cisplatin d(GpG) 1,2-intrastrand cross-link at the position denoted by asterisks, was determined in solution by high-resolution 2D NMR spectroscopy and restrained molecular dynamics refinement. The cis-[Pt(NH3)2 inverted question markd(GpG-N7(1), N7(2)) inverted question mark] lesion causes the adjacent guanine bases to roll toward one another by 49 degrees, leading to an overall helix bend angle of 78 degrees. These features are more exaggerated than those observed in the X-ray crystal structure determined for the same platinated duplex [Takahara et al. (1995) Nature 377, 649-652]. A common property of the solution and crystal structures is the widening and flattening of the minor groove opposite the platinum adduct, affording geometric parameters resembling those found in A-form DNA. This deformation is especially noteworthy for the solution structure because its sugar puckers are primarily those of B-form DNA. The unwinding of the helix at the site of platination is 25 degrees. The curvature and shape of the platinated duplex are remarkably similar to those observed in DNA duplexes complexed by the HMG-domain proteins SRY and LEF-1. The structure reveals how cisplatin binding alters DNA in such a manner as to facilitate HMG-domain protein recognition.
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The line below this paragraph, {{ABSTRACT_PUBMED_9649303}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9649303 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9649303}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A84 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A84 OCA].
==Reference==
==Reference==
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[[Category: Duplex]]
[[Category: Duplex]]
[[Category: Hmg-domain interaction]]
[[Category: Hmg-domain interaction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:20:52 2008''

Revision as of 13:20, 30 June 2008

Image:1a84.png

Template:STRUCTURE 1a84

NMR SOLUTION STRUCTURE OF A DNA DODECAMER DUPLEX CONTAINING A CIS-DIAMMINEPLATINUM(II) D(GPG) INTRASTRAND CROSS-LINK, THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN, 1 STRUCTURE

Template:ABSTRACT PUBMED 9649303

About this Structure

Full experimental information is available from OCA.

Reference

NMR solution structure of a DNA dodecamer duplex containing a cis-diammineplatinum(II) d(GpG) intrastrand cross-link, the major adduct of the anticancer drug cisplatin., Gelasco A, Lippard SJ, Biochemistry. 1998 Jun 30;37(26):9230-9. PMID:9649303

Page seeded by OCA on Mon Jun 30 16:20:52 2008

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