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1aci

From Proteopedia

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[[Image:1aci.jpg|left|200px]]
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[[Image:1aci.png|left|200px]]
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{{STRUCTURE_1aci| PDB=1aci | SCENE= }}
{{STRUCTURE_1aci| PDB=1aci | SCENE= }}
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'''L11 RIBOSOMAL PROTEIN RNA BINDING DOMAIN, NMR, 20 STRUCTURES'''
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===L11 RIBOSOMAL PROTEIN RNA BINDING DOMAIN, NMR, 20 STRUCTURES===
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==Overview==
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Ribosomal protein L11 interacts with a 58-nucleotide domain of large subunit ribosomal RNA; both the protein and its RNA target have been highly conserved. The antibiotic thiostrepton recognizes the same RNA domain, and binds to the ribosome cooperatively with L11. Experiments presented here show that RNA recognition and thiostrepton cooperativity can be attributed to C- and N-terminal domains of L11, respectively. Under trypsin digestion conditions that degrade Bacillus stearothermophilus L11 to small fragments, the target RNA protects the C-terminal 77 residues from digestion, and thiostrepton and RNA in combination protect the entire protein. A 76-residue C-terminal fragment of L11 was overexpressed and shown to fold into a stable structure binding ribosomal RNA with essentially the same properties as full-length L11. An L11.thiostrepton.RNA complex was 100-200-fold more stable than expected on the basis of L11-RNA and thiostrepton-RNA binding affinities; similar measurements with the C-terminal fragment detected no cooperativity with thiostrepton. L11 function is thus more complex than simple interaction with ribosomal RNA; we suggest that thiostrepton mimics some ribosomal component or factor that normally interacts with the L11 N-terminal domain.
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The line below this paragraph, {{ABSTRACT_PUBMED_8634289}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8634289 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8634289}}
==About this Structure==
==About this Structure==
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1ACI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACI OCA].
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1ACI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACI OCA].
==Reference==
==Reference==
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[[Category: L11-c76]]
[[Category: L11-c76]]
[[Category: Ribosomal protein]]
[[Category: Ribosomal protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:06:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:34:46 2008''

Revision as of 13:34, 30 June 2008

Image:1aci.png

Template:STRUCTURE 1aci

L11 RIBOSOMAL PROTEIN RNA BINDING DOMAIN, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 8634289

About this Structure

1ACI is a Single protein structure of sequence from Geobacillus stearothermophilus. Full experimental information is available from OCA.

Reference

Cooperative interactions of RNA and thiostrepton antibiotic with two domains of ribosomal protein L11., Xing Y, Draper DE, Biochemistry. 1996 Feb 6;35(5):1581-8. PMID:8634289

Page seeded by OCA on Mon Jun 30 16:34:46 2008

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