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| - | [[Image:1aec.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1aec| PDB=1aec | SCENE= }} | | {{STRUCTURE_1aec| PDB=1aec | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF ACTINIDIN-E-64 COMPLEX+'''
| + | ===CRYSTAL STRUCTURE OF ACTINIDIN-E-64 COMPLEX+=== |
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| - | ==Overview==
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| - | E-64, 1-(L-trans-epoxysuccinylleucylamino)-4-guanidinobutane, is a potent and highly selective irreversible inhibitor of cysteine proteases. The crystal structure of a complex of actinidin and E-64 has been determined at 1.86-A resolution by using the difference Fourier method and refined to an R-factor of 14.5%. The electron density map clearly shows that the C2 atom of the E-64 epoxide ring is covalently bonded to the S atom of the active-site cysteine 25. The charged carboxyl group of E-64 forms four H-bonds with the protein and thus may play an important role in favorably positioning the inhibitor molecule for nucleophilic attack by the active-site thiolate anion. The interaction features between E-64 and actinidin are very similar to those seen in the papain-E-64 complex; however, the amino-4-guanidinobutane group orients differently. The crystals of the actinidin-E-64 complex diffracted much better than the papain-E-64 complex, and consequently the present study provides more precise geometrical information on the binding of the inhibitor. Moreover, this study provides yet another confirmation that the binding of E-64 is at the S subsites and not at the S' subsites as has been previously proposed. The original actinidin structure has been revised using the new cDNA sequence information.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_1606141}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 1606141 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_1606141}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Varughese, K I.]] | | [[Category: Varughese, K I.]] |
| | [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:09:24 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:40:17 2008'' |
Revision as of 13:40, 30 June 2008
Template:STRUCTURE 1aec
CRYSTAL STRUCTURE OF ACTINIDIN-E-64 COMPLEX+
Template:ABSTRACT PUBMED 1606141
About this Structure
1AEC is a Single protein structure of sequence from Actinidia chinensis. Full crystallographic information is available from OCA.
Reference
Crystal structure of an actinidin-E-64 complex., Varughese KI, Su Y, Cromwell D, Hasnain S, Xuong NH, Biochemistry. 1992 Jun 9;31(22):5172-6. PMID:1606141
Page seeded by OCA on Mon Jun 30 16:40:17 2008
