1dt0
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(New page: 200px<br /><applet load="1dt0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dt0, resolution 2.1Å" /> '''CLONING, SEQUENCE, AN...)
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Revision as of 11:26, 20 November 2007
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CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS
Overview
The gene encoding the iron-dependent superoxide dismutase from Pseudomonas, ovalis was cloned from a genomic library and sequenced. The ORF differs, from the previously published protein sequence, which was used for the, original structure determination, at 16 positions. The differences include, three additional inserted residues, one deleted residue and 12 point, substitutions. The gene was subcloned and the recombinant protein, overexpressed, purified and crystallized in a trigonal space group. The, structure was determined by molecular replacement and was refined to 2.1 A, resolution.
About this Structure
1DT0 is a Single protein structure of sequence from Pseudomonas putida with FE as ligand. Active as Superoxide dismutase, with EC number 1.15.1.1 Full crystallographic information is available from OCA.
Reference
Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:11053832
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