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1agj

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[[Image:1agj.gif|left|200px]]
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{{STRUCTURE_1agj| PDB=1agj | SCENE= }}
{{STRUCTURE_1agj| PDB=1agj | SCENE= }}
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'''EPIDERMOLYTIC TOXIN A FROM STAPHYLOCOCCUS AUREUS'''
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===EPIDERMOLYTIC TOXIN A FROM STAPHYLOCOCCUS AUREUS===
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==Overview==
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BACKGROUND: Staphylococcal epidermolytic toxins A and B (ETA and ETB) are responsible for the staphylococcal scalded skin syndrome of newborn and young infants; this condition can appear just a few hours after birth. These toxins cause the disorganization and disruption of the region between the stratum spinosum and the stratum granulosum--two of the three cellular layers constituting the epidermis. The physiological substrate of ETA is not known and, consequently, its mode of action in vivo remains an unanswered question. Determination of the structure of ETA and its comparison with other serine proteases may reveal insights into ETA's catalytic mechanism. RESULTS: The crystal structure of staphylococcal ETA has been determined by multiple isomorphous replacement and refined at 1.7 A resolution with a crystallographic R factor of 0.184. The structure of ETA reveals it to be a new and unique member of the trypsin-like serine protease family. In contrast to other serine protease folds, ETA can be characterized by ETA-specific surface loops, a lack of cysteine bridges, an oxyanion hole which is not preformed, an S1 specific pocket designed for a negatively charged amino acid and an ETA-specific specific N-terminal helix which is shown to be crucial for substrate hydrolysis. CONCLUSIONS: Despite very low sequence homology between ETA and other trypsin-like serine proteases, the ETA crystal structure, together with biochemical data and site-directed mutagenesis studies, strongly confirms the classification of ETA in the Glu-endopeptidase family. Direct links can be made between the protease architecture of ETA and its biological activity.
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(as it appears on PubMed at http://www.pubmed.gov), where 9261066 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9261066}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:48:16 2008''

Revision as of 13:48, 30 June 2008

Image:1agj.png

Template:STRUCTURE 1agj

EPIDERMOLYTIC TOXIN A FROM STAPHYLOCOCCUS AUREUS

Template:ABSTRACT PUBMED 9261066

About this Structure

1AGJ is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The structure of Staphylococcus aureus epidermolytic toxin A, an atypic serine protease, at 1.7 A resolution., Cavarelli J, Prevost G, Bourguet W, Moulinier L, Chevrier B, Delagoutte B, Bilwes A, Mourey L, Rifai S, Piemont Y, Moras D, Structure. 1997 Jun 15;5(6):813-24. PMID:9261066

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