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| | {{STRUCTURE_1agl| PDB=1agl | SCENE= }} | | {{STRUCTURE_1agl| PDB=1agl | SCENE= }} |
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| - | '''STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX'''
| + | ===STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX=== |
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| - | ==Overview==
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| - | The application of detailed structural data bases has now culminated in the successful design of a new generation of bisanthracyclines that form ultratight DNA complexes [Chaires, J. B., Leng, F., Przewloka, T., Fokt, I., Ling, Y. H., Perez-Soler, R., & Priebe, W. (1997) J. Med. Chem. 40, 261-266]. Daunomycin dimers were designed to bind to DNA in complexes resembling those of monomers intercalated at adjacent sites. The goal of the work described here was to determine, with X-ray crystallography, if a potent member of this newly designed and synthesized class of bisanthracyclines (WP631) binds as intended. WP631 is composed of two daunomycin molecules, linked N3' to N3' by a xylyl group. We have solved the 2.2 A X-ray crystal structure of a complex of WP631 bound to [d(CGATCG)]2. We demonstrate, on a detailed molecular level, that the WP631 design strategy is a success. The structures of WP631 and two daunomycin molecules bound to [d(CGATCG)]2 provide the unprecedented opportunity for detailed comparison of mono- and bis-intercalated complexes of the same chromophore, allowing us to distinguish effects of mono-intercalation from those of bis-intercalation. Differences are focused primarily in the centers of the complexes. DNA unwinding and other helical distortions propagate more efficiently to the center of the WP631 complex than to the center of the daunomycin complex. | + | The line below this paragraph, {{ABSTRACT_PUBMED_9166763}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9166763 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9166763}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Double helix]] | | [[Category: Double helix]] |
| | [[Category: Right handed dna]] | | [[Category: Right handed dna]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:14:34 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:48:23 2008'' |
Revision as of 13:48, 30 June 2008
Template:STRUCTURE 1agl
STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX
Template:ABSTRACT PUBMED 9166763
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of a DNA-bisdaunomycin complex., Hu GG, Shui X, Leng F, Priebe W, Chaires JB, Williams LD, Biochemistry. 1997 May 20;36(20):5940-6. PMID:9166763
Page seeded by OCA on Mon Jun 30 16:48:23 2008
