1ai2

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[[Image:1ai2.gif|left|200px]]
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{{STRUCTURE_1ai2| PDB=1ai2 | SCENE= }}
{{STRUCTURE_1ai2| PDB=1ai2 | SCENE= }}
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'''ISOCITRATE DEHYDROGENASE COMPLEXED WITH ISOCITRATE, NADP+, AND CALCIUM (FLASH-COOLED)'''
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===ISOCITRATE DEHYDROGENASE COMPLEXED WITH ISOCITRATE, NADP+, AND CALCIUM (FLASH-COOLED)===
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==Overview==
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Small structural perturbations in the enzyme isocitrate dehydrogenase (IDH) were made in order to evaluate the contribution of precise substrate alignment to the catalytic power of an enzyme. The reaction trajectory of IDH was modified (i) after the adenine moiety of nicotinamide adenine dinucleotide phosphate was changed to hypoxanthine (the 6-amino was changed to 6-hydroxyl), and (ii) by replacing Mg2+, which has six coordinating ligands, with Ca2+, which has eight coordinating ligands. Both changes make large (10(-3) to 10(-5)) changes in the reaction velocity but only small changes in the orientation of the substrates (both distance and angle) as revealed by cryocrystallographic trapping of active IDH complexes. The results provide evidence that orbital overlap produced by optimal orientation of reacting orbitals plays a major quantitative role in the catalytic power of enzymes.
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The line below this paragraph, {{ABSTRACT_PUBMED_9211842}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9211842 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9211842}}
==About this Structure==
==About this Structure==
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[[Category: Nadp]]
[[Category: Nadp]]
[[Category: Phosphorylation]]
[[Category: Phosphorylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:17:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:53:40 2008''

Revision as of 13:53, 30 June 2008

Image:1ai2.png

Template:STRUCTURE 1ai2

ISOCITRATE DEHYDROGENASE COMPLEXED WITH ISOCITRATE, NADP+, AND CALCIUM (FLASH-COOLED)

Template:ABSTRACT PUBMED 9211842

About this Structure

1AI2 is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1ikb. Full crystallographic information is available from OCA.

Reference

Orbital steering in the catalytic power of enzymes: small structural changes with large catalytic consequences., Mesecar AD, Stoddard BL, Koshland DE Jr, Science. 1997 Jul 11;277(5323):202-6. PMID:9211842

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