1akx

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{{STRUCTURE_1akx| PDB=1akx | SCENE= }}
{{STRUCTURE_1akx| PDB=1akx | SCENE= }}
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'''HIV-2 TRANS ACTIVATING REGION RNA COMPLEX WITH ARGININAMIDE, NMR, MINIMIZED AVERAGE STRUCTURE'''
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===HIV-2 TRANS ACTIVATING REGION RNA COMPLEX WITH ARGININAMIDE, NMR, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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The trans-activating region (TAR) RNA-Tat protein interaction is important for activation of transciption in the human immunodeficiency virus (HIV). A model complex for this interaction composed of the two base bulge HIV-2 TAR and the amide derivative of arginine was studied by multidimensional heteronuclear NMR. Because of the improved spectral properties of the HIV-2 TAR complex, a larger number of NOEs in the bulge region were observed than in earlier studies of the HIV-1 TAR-argininamide complex. A total of 681 NOE distance restraints were collected and used to determine the solution structure of the HIV-2 TAR-argininamide complex. As observed in the previously proposed model from this lab, the two A-form stems co-axially stack and the critical U23 and the argininamide are located in the major groove. Model calculations including non-experimental restraints indicate that U23 is within hydrogen bonding distance to A27 consistent with the formation of a U x A x U base-triple. Base-triple formation helps open the major groove to increase the accessibility of G26 to hydrogen bond donors from the guanidinium group of argininamide. Argininamide binding is stabilized by stacking of the guanidinium group between the bases of A22 and U23, forming an argininamide sandwich.
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(as it appears on PubMed at http://www.pubmed.gov), where 9126842 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9126842}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKX OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKX OCA].
==Reference==
==Reference==
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[[Category: Protein-rna interaction]]
[[Category: Protein-rna interaction]]
[[Category: Transcriptional activation]]
[[Category: Transcriptional activation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:24:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:03:58 2008''

Revision as of 14:04, 30 June 2008

Image:1akx.png

Template:STRUCTURE 1akx

HIV-2 TRANS ACTIVATING REGION RNA COMPLEX WITH ARGININAMIDE, NMR, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9126842

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of the HIV-2 TAR-argininamide complex., Brodsky AS, Williamson JR, J Mol Biol. 1997 Apr 4;267(3):624-39. PMID:9126842

Page seeded by OCA on Mon Jun 30 17:03:58 2008

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