1apa

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[[Image:1apa.gif|left|200px]]
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{{STRUCTURE_1apa| PDB=1apa | SCENE= }}
{{STRUCTURE_1apa| PDB=1apa | SCENE= }}
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'''X-RAY STRUCTURE OF A POKEWEED ANTIVIRAL PROTEIN, CODED BY A NEW GENOMIC CLONE, AT 0.23 NM RESOLUTION. A MODEL STRUCTURE PROVIDES A SUITABLE ELECTROSTATIC FIELD FOR SUBSTRATE BINDING.'''
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===X-RAY STRUCTURE OF A POKEWEED ANTIVIRAL PROTEIN, CODED BY A NEW GENOMIC CLONE, AT 0.23 NM RESOLUTION. A MODEL STRUCTURE PROVIDES A SUITABLE ELECTROSTATIC FIELD FOR SUBSTRATE BINDING.===
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==Overview==
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We have determined the crystal structure of alpha-pokeweed antiviral protein, a member of ribosome-inactivating proteins, at 0.23 nm resolution, by the molecular-replacement method. The crystals belong to the space group P2(1)2(1)2 with unit-cell dimensions a = 4.71, b = 11.63 and c = 4.96 nm, and contain one protein molecule/asymmetric unit based on a crystal volume/unit protein molecular mass of 2.1 x 10(-3) nm3/Da. The crystallographic residual value was reduced to 17.2% (0.6-0.23 nm resolution) with root-mean-square deviations in bond lengths of 1.9 pm and bond angles of 2.2 degrees. The C alpha-C alpha distance map shows that alpha-pokeweed antiviral protein is composed of three modules, the N-terminal (Ala1-Leu76), the central (Tyr77-Lys185) and the C-terminal (Tyr186-Thr266) modules. The substrate-binding site is formed as a cleft between the central and C-terminal modules and all the active residues exist on the central module. The electrostatic potential around the substrate-binding site shows that the central and C-terminal module sides of this cleft have a negatively and a positively charged region, respectively. This charge distribution in the protein seems to provide a suitable interaction with the substrate rRNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 7925458 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7925458}}
==About this Structure==
==About this Structure==
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[[Category: Antiviral protein]]
[[Category: Antiviral protein]]
[[Category: Genomic clone]]
[[Category: Genomic clone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:32:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:20:55 2008''

Revision as of 14:20, 30 June 2008

Image:1apa.png

Template:STRUCTURE 1apa

X-RAY STRUCTURE OF A POKEWEED ANTIVIRAL PROTEIN, CODED BY A NEW GENOMIC CLONE, AT 0.23 NM RESOLUTION. A MODEL STRUCTURE PROVIDES A SUITABLE ELECTROSTATIC FIELD FOR SUBSTRATE BINDING.

Template:ABSTRACT PUBMED 7925458

About this Structure

1APA is a Single protein structure of sequence from Phytolacca americana. Full crystallographic information is available from OCA.

Reference

X-ray structure of a pokeweed antiviral protein, coded by a new genomic clone, at 0.23 nm resolution. A model structure provides a suitable electrostatic field for substrate binding., Ago H, Kataoka J, Tsuge H, Habuka N, Inagaki E, Noma M, Miyano M, Eur J Biochem. 1994 Oct 1;225(1):369-74. PMID:7925458

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