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| - | [[Image:1aqe.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1aqe| PDB=1aqe | SCENE= }} | | {{STRUCTURE_1aqe| PDB=1aqe | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE Y73E MUTANT OF CYTOCHROME C OF CLASS III (AMBLER) 26 KD'''
| + | ===CRYSTAL STRUCTURE OF THE Y73E MUTANT OF CYTOCHROME C OF CLASS III (AMBLER) 26 KD=== |
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| - | ==Overview==
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| - | A combination of structural, kinetic, and interaction experiments has been used to study the role of a highly conserved aromatic residue, Tyr73, parallel to the sixth heme axial ligand of heme 4 in multiheme cytochrome c3 (Mr = 26 000), also called cytochrome cc3 or octaheme cytochrome, from Desulfovibrio desulfuricans Norway. This residue is expected to be involved in intermolecular electron transfer and protein-protein interaction, since heme 4 is described to be the interaction site between physiological partners. The kinetic experiments show that the Y73E replacement provokes no significant change in the electron-transfer reaction with the physiological partner, the [NiFeSe] hydrogenase, but that the protein-protein interaction between cytochrome c3 (Mr = 26 000) and hydrogenase is strongly affected by the mutation. The aromatic residue does not play a role in maintaining the axial heme ligand in a particular orientation, since the mutation did not affect the orientation of histidine 77, the sixth axial ligand of heme 4. The structural analysis by X-ray crystallography clearly shows that a rearrangement of the charged residues in the vicinity of the mutation site is responsible for the change in protein-protein interaction, which is of an electrostatic nature. Lys22 and Arg66, residues which are located at the interacting surface, are twisted toward the mutated position Glu73 in order to compensate for the negative charge and therefore are no longer accessible for the docking with a physiological partner. Tyr73 has instead a structural function and probably a role in maintaining the hydrophobic environment of the heme 4 cavity rather than a function in the intermolecular electron transfer with the physiological partners.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9485359}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9485359 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9485359}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Octaheme cytochrome]] | | [[Category: Octaheme cytochrome]] |
| | [[Category: Point mutant]] | | [[Category: Point mutant]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:34:56 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:24:23 2008'' |
Revision as of 14:24, 30 June 2008
Template:STRUCTURE 1aqe
CRYSTAL STRUCTURE OF THE Y73E MUTANT OF CYTOCHROME C OF CLASS III (AMBLER) 26 KD
Template:ABSTRACT PUBMED 9485359
About this Structure
1AQE is a Single protein structure of sequence from Desulfovibrio desulfuricans. Full crystallographic information is available from OCA.
Reference
Structural and kinetic studies of the Y73E mutant of octaheme cytochrome c3 (Mr = 26 000) from Desulfovibrio desulfuricans Norway., Aubert C, Giudici-Orticoni MT, Czjzek M, Haser R, Bruschi M, Dolla A, Biochemistry. 1998 Feb 24;37(8):2120-30. PMID:9485359
Page seeded by OCA on Mon Jun 30 17:24:23 2008
