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1au7

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[[Image:1au7.gif|left|200px]]
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[[Image:1au7.png|left|200px]]
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{{STRUCTURE_1au7| PDB=1au7 | SCENE= }}
{{STRUCTURE_1au7| PDB=1au7 | SCENE= }}
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'''PIT-1 MUTANT/DNA COMPLEX'''
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===PIT-1 MUTANT/DNA COMPLEX===
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==Overview==
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Pit-1, a member of the POU domain family of transcription factors, characterized by a bipartite DNA-binding domain, serves critical developmental functions based on binding to diverse DNA elements in its target genes. Here we report a high resolution X-ray analysis of the Pit-1 POU domain bound to a DNA element as a homodimer. This analysis reveals that Pit-1 subdomains bind to perpendicular faces of the DNA, rather than opposite faces of the DNA as in Oct-1. This is accomplished by different spacing and orientation of the POU-specific domain. Contrary to previous predictions, the dimerization interface involves the carboxyl terminus of the DNA recognition helix of the homeodomain, which in an extended conformation interacts with specific residues at the amino terminus of helix alpha1 and in the loop between helices alpha3 and alpha4 of the POU-specific domain of the symmetry related monomer. These features suggest the molecular basis of disease-causing mutations in Pit-1 and provide potential basis for the flexible allostery between protein domains and DNA sites in the activation of target genes.
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(as it appears on PubMed at http://www.pubmed.gov), where 9009203 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9009203}}
==About this Structure==
==About this Structure==
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[[Category: Pou domain]]
[[Category: Pou domain]]
[[Category: Transcription factor]]
[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:41:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:36:10 2008''

Revision as of 14:36, 30 June 2008

Image:1au7.png

Template:STRUCTURE 1au7

PIT-1 MUTANT/DNA COMPLEX

Template:ABSTRACT PUBMED 9009203

About this Structure

1AU7 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility., Jacobson EM, Li P, Leon-del-Rio A, Rosenfeld MG, Aggarwal AK, Genes Dev. 1997 Jan 15;11(2):198-212. PMID:9009203

Page seeded by OCA on Mon Jun 30 17:36:10 2008

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